Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit

• Published in: Materials Science & Engineering C Vol. 32; no. 4; pp. 922 - 928
• Main Authors: Benhabiles, M.S., Abdi, N., Drouiche, N., Lounici, H., Pauss, A., Goosen, M.F.A., Mameri, N.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2012; Elsevier
• Subjects: Biochemical engineering; Bioreactor; Engineering Sciences; Environmental Sciences; Enzyme; Food processing; Life Sciences; Protein hydrolysate; Ultrafiltration; Protein hydrolysate; Bioreactor; Ultrafiltration; Food processing; Enzyme; Biochemical engineering
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2012.02.013

The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i. e. pH=1.5–2 and incubation time of 6h) could be satisfactory used for the enzymatic hydrolysis of fish solid waste. The optimal conditions for enzymatic reaction were: temperature 48°C, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200Lm−2bar−1. However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs. ► Evaluating to produce a (FPH) by enzymatic hydrolysis of sardine solid wastes was achieved. ► Investigation of key parameters for optimal conditions for enzymatic hydrolysis have been studied. ► Valorization of sardine waste was realized by enzymatic hydrolysis process. ► Performances of this enzyme gave comparable results to those obtained with commercial pepsin. ► The nutritional quality of the FPH produced appears to be satisfactory.