DNA substrate recognition and processing by the full-length human UPF1 helicase

• Published in: Nucleic acids research Vol. 45; no. 12; pp. 7354 - 7366
• Main Authors: Dehghani-Tafti, Saba, Sanders, Cyril M
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 07.07.2017
• Subjects: Base Sequence; Cloning, Molecular; DNA - chemistry; DNA - genetics; DNA - metabolism; DNA Replication; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression; Humans; Nonsense Mediated mRNA Decay; Nucleic Acid Conformation; Nucleic Acid Enzymes; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; RNA Stability; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - metabolism; Substrate Specificity; Trans-Activators - genetics; Trans-Activators - metabolism

UPF1 is a conserved helicase required for nonsense-mediated decay (NMD) regulating mRNA stability in the cytoplasm. Human UPF1 (hUPF1) is also needed for nuclear DNA replication. While loss of NMD is tolerated, loss of hUPF1 induces a DNA damage response and cell cycle arrest. We have analysed nucle...