A chemical-genetics approach to study the role of atypical Protein Kinase C in Drosophila

• Published in: Development (Cambridge) Vol. 146; no. 2
• Main Authors: Hannaford, Matthew, Loyer, Nicolas, Tonelli, Francesca, Zoltner, Martin, Januschke, Jens
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 15.01.2019
• Subjects: Alleles; Animals; Cell Division; Cell Polarity; Drosophila melanogaster - embryology; Drosophila melanogaster - enzymology; Drosophila melanogaster - genetics; Drosophila Proteins - antagonists & inhibitors; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Embryo, Nonmammalian - cytology; Embryo, Nonmammalian - metabolism; Larva - cytology; Larva - metabolism; Loss of Function Mutation - genetics; Neurons - metabolism; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - genetics; Protein Kinase C - metabolism; RNA Interference; Techniques and Resources; Chemical genetics; Neuroblasts; Drosophila; atypical Protein Kinase C; Asymmetric cell division

Studying the function of proteins using genetics in cycling cells is complicated by the fact that there is often a delay between gene inactivation and the time point of phenotypic analysis. This is particularly true when studying kinases that have pleiotropic functions and multiple substrates. neuro...