Single-cell analysis reveals CD34+CD90+ endothelial cells promote tumor metastasis in gallbladder cancer

Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however,...

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Published inNPJ precision oncology Vol. 9; no. 1; pp. 242 - 12
Main Authors Hou, Minghui, Chen, Jianan, Jiang, Youhai, Liu, Chunliang, Wang, Xiang, Liu, Erdong, Zong, Yali, Gu, Mingye, Meng, Zhengyuan, Wang, Senyan, Wei, Wenjuan, Liu, Qi, Wang, Hongyang, Fu, Jing, Zhao, Xiaofang
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 18.07.2025
Nature Publishing Group
Nature Portfolio
Subjects
692/699/67/322
692/699/67/327
Cancer Research
Cell growth
Extracellular matrix
Flow cytometry
Gallbladder cancer
Gene Therapy
Genes
Human Genetics
Immunoglobulins
Internal Medicine
Medical prognosis
Medicine
Medicine & Public Health
Metastasis
Oncology
Patients
Survival analysis
Vascular endothelial growth factor
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Abstract Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34 + CD90 + ECs (SAEndo2). The CD34 + CD90 + endothelial subset correlates with patients’ poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34 + CD90 + ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34 + CD90 + ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34 + CD90 + ECs’ promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.
AbstractList Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34 + CD90 + ECs (SAEndo2). The CD34 + CD90 + endothelial subset correlates with patients’ poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34 + CD90 + ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34 + CD90 + ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34 + CD90 + ECs’ promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.
Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34+CD90+ ECs (SAEndo2). The CD34+CD90+ endothelial subset correlates with patients’ poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34+CD90+ ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34+CD90+ ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34+CD90+ ECs’ promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.
Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34 CD90 ECs (SAEndo2). The CD34 CD90 endothelial subset correlates with patients' poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34 CD90 ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34 CD90 ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34 CD90 ECs' promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.
Abstract Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34+CD90+ ECs (SAEndo2). The CD34+CD90+ endothelial subset correlates with patients’ poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34+CD90+ ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34+CD90+ ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34+CD90+ ECs’ promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.
Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34+CD90+ ECs (SAEndo2). The CD34+CD90+ endothelial subset correlates with patients' poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34+CD90+ ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34+CD90+ ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34+CD90+ ECs' promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34+CD90+ ECs (SAEndo2). The CD34+CD90+ endothelial subset correlates with patients' poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34+CD90+ ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34+CD90+ ECs display enhanced activation of TGF-β signaling, and TGF-β inhibition abolishes the CD34+CD90+ ECs' promotion effect on GBC cell migration. Collectively, our study provides a detailed profiling of endothelial cells in GBC and identifies an essential endothelial population that regulates GBC metastasis, laying new theoretical insight and offering a potential therapeutic target for GBC metastasis.
ArticleNumber 242
Author Hou, Minghui
Zhao, Xiaofang
Gu, Mingye
Chen, Jianan
Liu, Chunliang
Zong, Yali
Liu, Erdong
Jiang, Youhai
Meng, Zhengyuan
Liu, Qi
Wei, Wenjuan
Wang, Xiang
Wang, Hongyang
Fu, Jing
Wang, Senyan
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  organization: Department of Translational Medicine Center, The First Affiliated Hospital of Zhengzhou University, International Cooperation Laboratory on Signal Transduction, National Center for Liver Cancer, Ministry of Education Key Laboratory on Signaling Regulation and Targeting Therapy of Liver Cancer, Shanghai Key Laboratory of Hepatobiliary Tumor Biology, Eastern Hepatobiliary Surgery Hospital, Naval Medical University
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  organization: Institute of Metabolism & Integrative Biology, Fudan University
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  organization: Department of Translational Medicine Center, The First Affiliated Hospital of Zhengzhou University
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  surname: Wei
  fullname: Wei, Wenjuan
  organization: Department of Translational Medicine Center, The First Affiliated Hospital of Zhengzhou University
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  surname: Wang
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  email: hywangk@vip.sina.com
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  givenname: Jing
  surname: Fu
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  email: fujing-724@163.com
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Cites_doi 10.1016/j.semcancer.2023.04.007
10.3390/cancers13040733
10.1038/s41421-022-00445-8
10.1016/j.cell.2013.06.037
10.6004/jnccn.2021.0022
10.1007/s00441-011-1222-6
10.1038/s41556-020-00613-6
10.1016/j.cmet.2020.03.009
10.1016/j.bbamcr.2013.02.013
10.1136/jitc-2020-000564
10.1093/nsr/nwae231
10.1038/s41586-019-1631-3
10.1158/1078-0432.CCR-17-2653
10.1084/jem.20120662
10.1016/j.pharmthera.2022.108211
10.1038/ncomms11853
10.1038/s43018-020-00147-8
10.1038/nrc3628
10.1161/CIRCULATIONAHA.122.063075
10.1016/j.molcel.2017.01.023
10.1186/s13045-023-01487-5
10.1016/j.jhep.2021.06.023
10.1016/j.ccell.2020.03.002
10.1016/j.cell.2020.01.015
10.1038/s41572-022-00398-y
10.1038/s41467-022-33052-y
10.1016/j.annonc.2020.07.009
10.1186/s13046-023-02714-0
10.1016/S0140-6736(21)00153-7
10.1038/s42255-019-0102-3
10.1038/s41419-024-06800-9
10.7150/thno.71833
10.1016/j.jtho.2020.03.003
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References V Geldhof (1040_CR13) 2022; 13
M Kong (1040_CR19) 2013; 1833
MT Bu (1040_CR32) 2022; 240
C Ziegenhain (1040_CR5) 2017; 65
M Fan (1040_CR24) 2023; 9
HA Trau (1040_CR18) 2016; 31
1040_CR3
JC Roa (1040_CR1) 2022; 8
K Rohlenova (1040_CR10) 2020; 31
S Chen (1040_CR15) 2021; 23
PY Chen (1040_CR30) 2019; 1
JW Valle (1040_CR4) 2021; 397
J Goveia (1040_CR20) 2020; 37
D Ciardiello (1040_CR33) 2020; 31
AB Benson (1040_CR2) 2021; 19
Z Wu (1040_CR31) 2024; 15
P Chen (1040_CR7) 2021; 11
W Ma (1040_CR9) 2021; 2
1040_CR16
P Ramachandran (1040_CR22) 2019; 575
C Anderberg (1040_CR27) 2013; 210
N Reymond (1040_CR12) 2013; 13
J Kalucka (1040_CR21) 2020; 180
T Watabe (1040_CR11) 2023; 92
L Paz-Ares (1040_CR34) 2020; 15
K De Bock (1040_CR23) 2013; 154
X Wang (1040_CR8) 2022; 8
SM Evrard (1040_CR28) 2016; 7
1040_CR37
LA van Meeteren (1040_CR29) 2012; 347
JA Waters (1040_CR17) 2022; 12
Y Li (1040_CR14) 2022; 12
M Niu (1040_CR38) 2023; 16
J Strauss (1040_CR35) 2018; 24
H Luo (1040_CR39) 2022; 13
Y Zhang (1040_CR6) 2021; 75
WF Wei (1040_CR26) 2023; 42
Y Dong (1040_CR25) 2023; 147
C Yoo (1040_CR36) 2023; 78
References_xml – volume: 92
  start-page: 130
  year: 2023
  ident: 1040_CR11
  publication-title: Semin. Cancer Biol.
  doi: 10.1016/j.semcancer.2023.04.007
– volume: 31
  start-page: 436
  year: 2016
  ident: 1040_CR18
  publication-title: Hum. Reprod.
– ident: 1040_CR3
  doi: 10.3390/cancers13040733
– volume: 78
  start-page: 758
  year: 2023
  ident: 1040_CR36
  publication-title: Hepatology
– volume: 8
  start-page: 101
  year: 2022
  ident: 1040_CR8
  publication-title: Cell Discov.
  doi: 10.1038/s41421-022-00445-8
– volume: 154
  start-page: 651
  year: 2013
  ident: 1040_CR23
  publication-title: Cell
  doi: 10.1016/j.cell.2013.06.037
– volume: 19
  start-page: 541
  year: 2021
  ident: 1040_CR2
  publication-title: J. Natl Compr. Canc Netw.
  doi: 10.6004/jnccn.2021.0022
– volume: 347
  start-page: 177
  year: 2012
  ident: 1040_CR29
  publication-title: Cell Tissue Res.
  doi: 10.1007/s00441-011-1222-6
– volume: 23
  start-page: 87
  year: 2021
  ident: 1040_CR15
  publication-title: Nat. Cell Biol.
  doi: 10.1038/s41556-020-00613-6
– volume: 31
  start-page: 862
  year: 2020
  ident: 1040_CR10
  publication-title: Cell Metab.
  doi: 10.1016/j.cmet.2020.03.009
– volume: 1833
  start-page: 1409
  year: 2013
  ident: 1040_CR19
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbamcr.2013.02.013
– ident: 1040_CR37
  doi: 10.1136/jitc-2020-000564
– ident: 1040_CR16
  doi: 10.1093/nsr/nwae231
– volume: 575
  start-page: 512
  year: 2019
  ident: 1040_CR22
  publication-title: Nature
  doi: 10.1038/s41586-019-1631-3
– volume: 24
  start-page: 1287
  year: 2018
  ident: 1040_CR35
  publication-title: Clin. Cancer Res.
  doi: 10.1158/1078-0432.CCR-17-2653
– volume: 210
  start-page: 563
  year: 2013
  ident: 1040_CR27
  publication-title: J. Exp. Med.
  doi: 10.1084/jem.20120662
– volume: 240
  start-page: 108211
  year: 2022
  ident: 1040_CR32
  publication-title: Pharmacol. Therapeut.
  doi: 10.1016/j.pharmthera.2022.108211
– volume: 7
  year: 2016
  ident: 1040_CR28
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms11853
– volume: 2
  start-page: 83
  year: 2021
  ident: 1040_CR9
  publication-title: Nat. Cancer
  doi: 10.1038/s43018-020-00147-8
– volume: 13
  start-page: 858
  year: 2013
  ident: 1040_CR12
  publication-title: Nat. Rev. Cancer
  doi: 10.1038/nrc3628
– volume: 147
  start-page: 669
  year: 2023
  ident: 1040_CR25
  publication-title: Circulation
  doi: 10.1161/CIRCULATIONAHA.122.063075
– volume: 65
  start-page: 631
  year: 2017
  ident: 1040_CR5
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2017.01.023
– volume: 11
  year: 2021
  ident: 1040_CR7
  publication-title: Clin. Transl. Med.
– volume: 16
  start-page: 94
  year: 2023
  ident: 1040_CR38
  publication-title: J. Hematol. Oncol.
  doi: 10.1186/s13045-023-01487-5
– volume: 12
  year: 2022
  ident: 1040_CR17
  publication-title: Front. Oncol.
– volume: 75
  start-page: 1128
  year: 2021
  ident: 1040_CR6
  publication-title: J. Hepatol.
  doi: 10.1016/j.jhep.2021.06.023
– volume: 37
  start-page: 421
  year: 2020
  ident: 1040_CR20
  publication-title: Cancer Cell
  doi: 10.1016/j.ccell.2020.03.002
– volume: 180
  start-page: 764
  year: 2020
  ident: 1040_CR21
  publication-title: Cell
  doi: 10.1016/j.cell.2020.01.015
– volume: 8
  start-page: 69
  year: 2022
  ident: 1040_CR1
  publication-title: Nat. Rev. Dis. Prim.
  doi: 10.1038/s41572-022-00398-y
– volume: 13
  year: 2022
  ident: 1040_CR13
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-022-33052-y
– volume: 31
  start-page: 1336
  year: 2020
  ident: 1040_CR33
  publication-title: Ann. Oncol.
  doi: 10.1016/j.annonc.2020.07.009
– volume: 42
  start-page: 160
  year: 2023
  ident: 1040_CR26
  publication-title: J. Exp. Clin. Cancer Res.
  doi: 10.1186/s13046-023-02714-0
– volume: 397
  start-page: 428
  year: 2021
  ident: 1040_CR4
  publication-title: Lancet
  doi: 10.1016/S0140-6736(21)00153-7
– volume: 1
  start-page: 912
  year: 2019
  ident: 1040_CR30
  publication-title: Nat. Metab.
  doi: 10.1038/s42255-019-0102-3
– volume: 15
  start-page: 422
  year: 2024
  ident: 1040_CR31
  publication-title: Cell Death Dis.
  doi: 10.1038/s41419-024-06800-9
– volume: 12
  start-page: 3818
  year: 2022
  ident: 1040_CR14
  publication-title: Theranostics
  doi: 10.7150/thno.71833
– volume: 13
  year: 2022
  ident: 1040_CR39
  publication-title: Nat. Commun.
– volume: 15
  start-page: 1210
  year: 2020
  ident: 1040_CR34
  publication-title: J. Thorac. Oncol.
  doi: 10.1016/j.jtho.2020.03.003
– volume: 9
  year: 2023
  ident: 1040_CR24
  publication-title: Sci. Adv.
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Snippet Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor...
Abstract Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor...
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SubjectTerms 692/699/67/322
692/699/67/327
Cancer Research
Cell growth
Extracellular matrix
Flow cytometry
Gallbladder cancer
Gene Therapy
Genes
Human Genetics
Immunoglobulins
Internal Medicine
Medical prognosis
Medicine
Medicine & Public Health
Metastasis
Oncology
Patients
Survival analysis
Vascular endothelial growth factor
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Title Single-cell analysis reveals CD34+CD90+ endothelial cells promote tumor metastasis in gallbladder cancer
URI https://link.springer.com/article/10.1038/s41698-025-01040-2
https://www.ncbi.nlm.nih.gov/pubmed/40681742
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Volume 9
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