Pseudotype Neutralization Assays: From Laboratory Bench to Data Analysis

• Published in: Methods and protocols Vol. 1; no. 1; p. 8
• Main Authors: Ferrara, Francesca, Temperton, Nigel
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 22.01.2018
• Subjects: Protocol; influenza; pseudotypes; antibodies; neutralization; lentiviral vector; serology
• ISSN: 2409-9279; 2409-9279
• DOI: 10.3390/mps1010008

Pseudotype neutralization assays are powerful tools to study functional antibody responses against viruses in low biosafety laboratories. However, protocols described in the literature differ widely with respect to material, reagents, and methods used to perform these assays and to analyse the raw d...