Dissecting lipid droplet biology with coherent Raman scattering microscopy

Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated with a variety of diseases. The content level, compo...

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Bibliographic Details
Published inJournal of cell science Vol. 135; no. 5
Main Authors Chen, Tao, Yavuz, Ahmet, Wang, Meng C.
Format Journal Article
LanguageEnglish
Published England The Company of Biologists Ltd 01.03.2022
Subjects
Biology
Imaging
Lipid Droplets
Lipids
Microscopy
Review
Spectrum Analysis, Raman
Coherent Raman scattering microscopy
Lipid droplet
Lipid metabolism
Label-free imaging
Genetic screens
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Summary:Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated with a variety of diseases. The content level, composition and mobility of LDs are crucial for their physiological and pathological functions, and these different parameters of LDs are subject to regulation by genetic factors and environmental inputs. Coherent Raman scattering (CRS) microscopy utilizes optical nonlinear processes to probe the intrinsic chemical bond vibration, offering label-free, quantitative imaging of lipids in vivo with high chemical specificity and spatiotemporal resolution. In this Review, we provide an overview over the principle of CRS microscopy and its application in tracking different parameters of LDs in live cells and organisms. We also discuss the use of CRS microscopy in genetic screens to discover lipid regulatory mechanisms and in understanding disease-related lipid pathology.
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These authors contributed equally to this work
ISSN:0021-9533
1477-9137
1477-9137
DOI:10.1242/jcs.252353