Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene

• Published in: Diagnostic microbiology and infectious disease Vol. 79; no. 2; pp. 240 - 241
• Main Authors: Caldas, Paulo Cesar de Souza, Campos, Carlos Eduardo Dias, dos Reis, Lusiano Motta, Ferreira, Nicole Victor, de Carvalho, Luciana Distásio, da Silva, Mariza Villas Boas, Medeiros, Reginalda Ferreira de Melo, Montes, Fátima Cristina Onofre Fandinho, Ramos, Jesus Pais
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2014
• Subjects: Bacterial Proteins - genetics; Brazil; Chaperonin 60 - genetics; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Humans; Infectious Disease; Internal Medicine; Molecular Sequence Data; Molecular Typing; Mycobacterium avium Complex - classification; Mycobacterium avium Complex - genetics; Mycobacterium avium Complex - isolation & purification; Mycobacterium avium-intracellulare Infection - microbiology; Mycobacterium intracellulare; Nontuberculous mycobacteria; Phylogeny; Pneumonia - microbiology; Polymerase Chain Reaction; PRA-hsp65; Restriction Mapping; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sputum - microbiology; Brazil; Mycobacterium intracellulare; PRA- hsp65; Nontuberculous mycobacteria; PRA-hsp65
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2014.01.028

Abstract This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare . We report a mutation at position 453 creating a new Hae III cutting site and, therefore, a new PRA- hsp65 M. intracellulare profile.