tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci

• Published in: Nucleic acids research Vol. 45; no. 21; p. e178
• Main Authors: Ott, Alina, Liu, Sanzhen, Schnable, James C., Yeh, Cheng-Ting ‘Eddy’, Wang, Kai-Sin, Schnable, Patrick S.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.12.2017
• Subjects: Methods Online

Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orient...