Luminescence lifetime imaging of three-dimensional biological objects

• Published in: Journal of cell science Vol. 134; no. 9; pp. 1 - 17
• Main Authors: Dmitriev, Ruslan I., Intes, Xavier, Barroso, Margarida M.
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 01.05.2021
• Series: Subject Collection: Imaging
• Subjects: Biosensing Techniques; Fluorescence Resonance Energy Transfer; Imaging; Luminescence; Optical Imaging; Oxygen; Review; Imaging; Fluorescence lifetime; Three dimensional

A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical tools enable multiparameter quantitative imaging of live and fixed cells, three-dimensional (3D) systems, tissues, organs a...