A variant OSR1 allele which disturbs OSR1 mRNA expression in renal progenitor cells is associated with reduction of newborn kidney size and function

• Published in: Human molecular genetics Vol. 20; no. 21; pp. 4167 - 4174
• Main Authors: Zhang, Zhao, Iglesias, Diana, Eliopoulos, Nicoletta, El Kares, Reyhan, Chu, LeeLee, Romagnani, Paola, Goodyer, Paul
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.2011
• Subjects: Adult; Alleles; Animals; Animals, Newborn; Base Sequence; Biological and medical sciences; Cell Separation; Cord blood; cystatin C; Cystatin C - metabolism; Enhancers; Fetuses; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Developmental; Genetics of eukaryotes. Biological and molecular evolution; Genotypes; Heterozygote; Humans; In Situ Hybridization; Infant, Newborn; Kidney; Kidney - enzymology; Kidney - growth & development; Kidney - pathology; Mesenchymal Stromal Cells - enzymology; Mesenchyme; Mice; Molecular and cellular biology; Molecular Sequence Data; Mutation - genetics; Neonates; Nephrons; Nucleic Acid Conformation; Organ Size; Pax2 protein; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Renal function; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem cells; Transcription; Transcription Factors - genetics; Transcription Factors - metabolism; Tumors; Umbilical Cord - metabolism; Ureter; Wilms Tumor - enzymology; Wilms Tumor - genetics; Wilms Tumor - pathology; Human; Renal function; Genetic variability; Stem cell; Genotype; Kidney; Variant; Allele; Newborn; Messenger RNA; Urinary system; Genetics; Progenitor cell
• DOI: 10.1093/hmg/ddr341
• ISSN: 0964-6906; 1460-2083

Human nephrons are formed during fetal life through an interaction between the branching ureteric bud and progenitor cells. The wide variation in final nephron number has been attributed to allelic variants of genes regulating ureteric bud arborization. Here, we hypothesize that dysfunctional variants of the Odd-Skipped Related 1 (OSR1) gene which compromise the renal progenitor cell pool might also limit newborn kidney size and function. We show that OSR1 is expressed in human mesenchymal stem cells, the blastemal component of Wilms tumors and CD24+/CD133+ progenitor cells isolated from the mature kidney. We identified an OSR1 rs12329305(T) allele in 6% of normal Caucasians which alters an exon2 splice enhancer. This variant is predicted to reduce spliceosome-binding affinity and stability of the OSR1 mRNA. In cultured cells, the OSR1 rs12329305 (T) allele produced no identifiable transcript. Normal Caucasian newborns from Montreal with the OSR1 rs12329305 (T) allele had kidney volume 11.8% smaller (P= 0.006) and cord blood cystatin C levels 12.6% higher (P = 0.005) than those with wild-type genotype. Effects of the OSR1 rs12329305 (T) allele are additive with genes that alter ureteric bud branching. Kidney volume was reduced more in newborns bearing both RET rs1800860 (A) and OSR1 rs12329305 (T) alleles (22%, P= 0.0008) and cystatin C was increased by 17% (P= 0.006) versus newborns with wild-type alleles. Although only two subjects had PAX2 rs11599825 (A) and OSR1 rs12329305 (T) alleles, kidney size was reduced by 27% and cystatin C was increased by 14% versus wild-types (P= NS).