Neutrophil chemokines in cultured nasal fibroblasts

• Published in: Allergy (Copenhagen) Vol. 57; no. 12; pp. 1159 - 1164
• Main Authors: Rudack, C., Hermann, W., Eble, J., Schroeder, J.‐M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Munksgaard International Publishers 01.12.2002; Blackwell
• Subjects: Allergic diseases; Antimicrobial Cationic Peptides; biological activity; Biological and medical sciences; Blood Proteins - pharmacology; Carrier Proteins - pharmacology; Cell Movement - drug effects; Cell Movement - immunology; Cells, Cultured; Chemokine CXCL1; chemokines; Chemokines - biosynthesis; Chemokines - immunology; Chemokines, CXC - immunology; Chemokines, CXC - metabolism; Chemotactic Factors - biosynthesis; Chemotactic Factors - immunology; Chromatography; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Fibroblasts - immunology; Fibroblasts - metabolism; Humans; Immunopathology; Intercellular Signaling Peptides and Proteins - biosynthesis; Intercellular Signaling Peptides and Proteins - immunology; Interleukin-8 - biosynthesis; Interleukin-8 - immunology; Interleukin‐8; Medical sciences; nasal fibroblast; Nasal Mucosa - cytology; Nasal Mucosa - immunology; Nasal Mucosa - metabolism; Neutrophils - immunology; Neutrophils - metabolism; Polymerase Chain Reaction; Respiratory and ent allergic diseases; RNA, Messenger - drug effects; RNA, Messenger - immunology; RNA, Messenger - metabolism; Stimulation, Chemical; Time Factors; Tumor Necrosis Factor-alpha - administration & dosage; Up-Regulation - drug effects; Up-Regulation - physiology; Human; Cell culture; Pathogenesis; Cytokine; chemokines; Interleukin-8; Biological activity; Sinusitis; Chemokine; ENT disease; Paranasal sinus disease; Molecular biology; Nasal fossa; Fibroblast; Reverse transcription polymerase chain reaction; ELISA assay; Interleukin 8; nasal fibroblast

Background: To gain insight into the mechanisms responsible for tissue neutrophil immigration in sinusitis, primary nasal fibroblasts are analyzed for synthesizing and delivering neutrophil chemokines. Methods: Primary nasal fibroblast cell culture was treated with tumor necrosis factor (TNF)‐α conc...