Role of CXCR4‐mediated bone marrow colonization in CNS infiltration by T cell acute lymphoblastic leukemia

• Published in: Journal of leukocyte biology Vol. 99; no. 6; pp. 1077 - 1087
• Main Authors: Jost, Tanja Rezzonico, Borga, Chiara, Radaelli, Enrico, Romagnani, Andrea, Perruzza, Lisa, Omodho, Lorna, Cazzaniga, Giovanni, Biondi, Andrea, Indraccolo, Stefano, Thelen, Marcus, Kronnie, Geertruy, Grassi, Fabio
• Format: Journal Article
• Language: English
• Published: United States 01.06.2016
• Subjects: Adolescent; Animals; blood‐brain barrier; Bone Marrow - drug effects; Bone Marrow - pathology; CCR6; CCR7; Cell Line, Transformed; Cell Line, Tumor; Central Nervous System - drug effects; Central Nervous System - pathology; Child; Child, Preschool; Female; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - metabolism; Heterocyclic Compounds - pharmacology; Humans; Liver - cytology; Liver - embryology; Male; meninges; Meninges - drug effects; Meninges - pathology; Mice; neuropathology; Phenotype; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology; Receptors, Chemokine - metabolism; Receptors, CXCR4 - antagonists & inhibitors; Receptors, CXCR4 - metabolism; Receptors, Notch - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism; Xenograft Model Antitumor Assays; blood-brain barrier; CCR7; CCR6; neuropathology; meninges
• DOI: 10.1189/jlb.5MA0915-394R
• ISSN: 0741-5400

CXCR4 in T‐ALL cells promotes BM colonization, a prelude to meninges invasion and potential pharmacological target for this leukemia. Infiltration of the central nervous system is a severe trait of T cell acute lymphoblastic leukemia. Inhibition of CXC chemokine receptor 4 significantly ameliorates T cell acute lymphoblastic leukemia in murine models of the disease; however, signaling by CXC chemokine receptor 4 is important in limiting the divagation of peripheral blood mononuclear cells out of the perivascular space into the central nervous system parenchyma. Therefore, Inhibition of CXC chemokine receptor 4 potentially may untangle T cell acute lymphoblastic leukemia cells from retention outside the brain. Here, we show that leukemic lymphoblasts massively infiltrate cranial bone marrow, with diffusion to the meninges without invasion of the brain parenchyma, in mice that underwent xenotransplantation with human T cell acute lymphoblastic leukemia cells or that developed leukemia from transformed hematopoietic progenitors. We tested the hypothesis that T cell acute lymphoblastic leukemia neuropathology results from meningeal infiltration through CXC chemokine receptor 4–mediated bone marrow colonization. Inhibition of leukemia engraftment in the bone marrow by pharmacologic CXC chemokine receptor 4 antagonism significantly ameliorated neuropathologic aspects of the disease. Genetic deletion of CXCR4 in murine hematopoietic progenitors abrogated leukemogenesis induced by constitutively active Notch1, whereas lack of CCR6 and CCR7, which have been shown to be involved in T cell and leukemia extravasation into the central nervous system, respectively, did not influence T cell acute lymphoblastic leukemia development. We hypothesize that lymphoblastic meningeal infiltration as a result of bone marrow colonization is responsible for the degenerative alterations of the neuroparenchyma as well as the alteration of cerebrospinal fluid drainage in T cell acute lymphoblastic leukemia xenografts. Therefore, CXC chemokine receptor 4 may constitute a pharmacologic target for T cell acute lymphoblastic leukemia neuropathology.