Stimulation of matrix formation in rabbit chondrocyte cultures by ascorbate. 1. Effect of ascorbate analogs and beta-aminopropionitrile

• Published in: Journal of orthopaedic research Vol. 6; no. 3; p. 397
• Main Authors: Wright, Jr, G C, Wei, X Q, McDevitt, C A, Lane, B P, Sokoloff, L
• Format: Journal Article
• Language: English
• Published: United States 01.05.1988
• Subjects: Aminopropionitrile - pharmacology; Animals; Ascorbic Acid - pharmacology; Bone Matrix - drug effects; Bone Matrix - metabolism; Cartilage, Articular - cytology; Cartilage, Articular - drug effects; Cartilage, Articular - metabolism; Cells, Cultured; Proteoglycans - biosynthesis; Rabbits

The most consistent effects of 0.2 mM L-ascorbate on monolayer cultures of rabbit articular chondrocytes were a diversion of incorporated radiosulfate into a pericellular matrix and enhancement of cell proliferation. Only with certain batches of fetal bovine serum (FBS) was there a cell-for-cell inc...