An Efficient Micropropagation Protocol for the Endangered European Shrub February Daphne (Daphne mezereum L.) and Identification of Bacteria in Culture

Daphne mezereum of the Thymelaeaceae family is a medicinal shrub occurring naturally in Europe and under legal protection in Poland. In the present study, a protocol developed for mass propagation of February daphne from nodal explants is presented. Micropropagation is one of the in vitro techniques...

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Published inAgriculture (Basel) Vol. 13; no. 9; p. 1692
Main Authors Nowakowska, Karolina, Nongdam, Potshangbam, Samsurizal, Nabilah Amany, Pacholczak, Andrzej
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2023
Subjects
Bacteria
Bacterial infections
Butyric acid
Cytokinins
Daphne mezereum
Endophytes
endophytic bacteria
Explants
Gram-positive bacteria
in vitro
In vitro methods and tests
Indole-3-butyric acid
Leaves
Life sciences
medicinal plant
Microorganisms
Micropropagation
Perlite
Pharmaceutical industry
plant growth regulators
Plant propagation
Propagation
Raw materials
Semisolids
Shoots
Woody plants
Poland
Netherlands
United States
Germany
United States > US
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Abstract Daphne mezereum of the Thymelaeaceae family is a medicinal shrub occurring naturally in Europe and under legal protection in Poland. In the present study, a protocol developed for mass propagation of February daphne from nodal explants is presented. Micropropagation is one of the in vitro techniques that allow the preservation of rare and valuable plants by developing efficient methods for their propagation. In the proliferation stage, explants were cultured in the Woody Plant Medium (WPM) with different cytokinins, and in the rooting stage on the semi-solid WPM medium with perlite, indole-3-butyric acid (IBA) in various concentrations was used. The maximum proliferation rate (five shoots per explant) was observed on the medium containing 4.14 μM of meta-Topolin (mT). When the regenerated shoots were rooted in vitro in the presence of IBA in various concentrations, that of 19.68 μM induced the highest number of roots per shoot (6.63) and the maximal root length (2.15 cm). It is also worth remembering that plants are often colonized by different groups of microorganisms, which also affect the diversity of the ecosystem. The endophytic bacteria inhabiting the D. mezereum shoots are Mycobacterium.
AbstractList Daphne mezereum of the Thymelaeaceae family is a medicinal shrub occurring naturally in Europe and under legal protection in Poland. In the present study, a protocol developed for mass propagation of February daphne from nodal explants is presented. Micropropagation is one of the in vitro techniques that allow the preservation of rare and valuable plants by developing efficient methods for their propagation. In the proliferation stage, explants were cultured in the Woody Plant Medium (WPM) with different cytokinins, and in the rooting stage on the semi-solid WPM medium with perlite, indole-3-butyric acid (IBA) in various concentrations was used. The maximum proliferation rate (five shoots per explant) was observed on the medium containing 4.14 μM of meta-Topolin (mT). When the regenerated shoots were rooted in vitro in the presence of IBA in various concentrations, that of 19.68 μM induced the highest number of roots per shoot (6.63) and the maximal root length (2.15 cm). It is also worth remembering that plants are often colonized by different groups of microorganisms, which also affect the diversity of the ecosystem. The endophytic bacteria inhabiting the D. mezereum shoots are Mycobacterium.
Audience Academic
Author Pacholczak, Andrzej
Samsurizal, Nabilah Amany
Nowakowska, Karolina
Nongdam, Potshangbam
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  surname: Pacholczak
  fullname: Pacholczak, Andrzej
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Snippet Daphne mezereum of the Thymelaeaceae family is a medicinal shrub occurring naturally in Europe and under legal protection in Poland. In the present study, a...
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SubjectTerms Bacteria
Bacterial infections
Butyric acid
Cytokinins
Daphne mezereum
Endophytes
endophytic bacteria
Explants
Gram-positive bacteria
in vitro
In vitro methods and tests
Indole-3-butyric acid
Leaves
Life sciences
medicinal plant
Microorganisms
Micropropagation
Perlite
Pharmaceutical industry
plant growth regulators
Plant propagation
Propagation
Raw materials
Semisolids
Shoots
Woody plants
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Title An Efficient Micropropagation Protocol for the Endangered European Shrub February Daphne (Daphne mezereum L.) and Identification of Bacteria in Culture
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https://doaj.org/article/b3842912c940452a9d50669efe1a93bb
Volume 13
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