The regulatory roles of Fasciola hepatica GSTO1 protein in inflammatory cytokine expression and apoptosis in murine macrophages

• Published in: Acta tropica Vol. 245; p. 106977
• Main Authors: Xifeng, Wang, Jiahua, Zhang, Ningxing, Li, Guowu, Zhang, Yunxia, Shang, Xuepeng, Cai, Jun, Qiao, Xianzhu, Xia, Qingling, Meng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2023
• Subjects: Anti-inflammatory; Apoptosis; Fasciola hepatica; Glutathione s-transferase; NF-κB, MAPKs; Fasciola hepatica; Glutathione s-transferase; Anti-inflammatory; NF-κB, MAPKs; Apoptosis
• DOI: 10.1016/j.actatropica.2023.106977
• ISSN: 0001-706X

•F. hepatica recombinant GSTO1 protein exhibited potent resistance to oxidative damage.•F. hepatica recombinant GSTO1 protein could induce apoptosis in murine macrophage RAW264.7.•F. hepatica recombinant GSTO1 protein suppressed LPS-induced NF-κB and MAPK pathway (p38, ERK, JNK) activation in murine macrophage RAW264.7. Fascioliasis, a global zoonotic parasitic disease, is mainly caused by Fasciola hepatica (F. hepatica) parasitizing in the livers of hosts, mainly humans and herbivores. Glutathione S-transferase (GST) is one of the important excretory- secretory products (ESPs) from F. hepatica, however, the regulatory roles of its Omega subtype in the immunomodulatory effects remain unknown. Here, we expressed F. hepatica recombinant GSTO1 protein (rGSTO1) in Pichia pastoris and analyzed its antioxidant properties. Then, the interaction between F. hepatica rGSTO1 and RAW264.7 macrophages and its effects on inflammatory responses and cell apoptosis were further explored. The results revealed that GSTO1 of F. hepatica owned the potent ability to resist oxidative stress. F. hepatica rGSTO1 could interact with RAW264.7 macrophages and inhibit its cell viability, furthermore, it may suppress the production of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α, but promote the expression of anti-inflammatory cytokine IL-10. In addition, F. hepatica rGSTO1 may down-regulate the ratio of Bcl-2/Bax, and increase the expression of pro-apoptotic protein caspase-3, thereby eliciting the apoptosis of macrophages. Notably, F. hepatica rGSTO1 inhibited the activation of nuclear factor-κB (NF-κB) and mitogen‑activated protein kinases (MAPKs p38, ERK and JNK) pathways in LPS-activated RAW264.7 cells, exerting potent modulatory effects on macrophages. These findings suggested that F. hepatica GSTO1 can modulate the host immune response, which provided new insights into the immune evasion mechanism of F. hepatica infection in host.