Application of a biosensor for super-sensitive detector of clenbuterol

• Published in: New Zealand journal of agricultural research Vol. 50; no. 5; pp. 689 - 695
• Main Authors: Cunshe, Chen, Xiaojuan, Li
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01.12.2007
• Subjects: ATPase; biosensor; molecular motors
• ISSN: 0028-8233; 1175-8775
• DOI: 10.1080/00288230709510339

We explored the potential of the motor protein F 0 F 1 -ATPase as biosensors of harmful residues in food. A fluorescence probe F1300 labelled in inner chromatophores was used as a pH indicator for detecting proton flux driven by ATP synthesis in F 0 F 1 -ATPase. Furthermore, the F 1 β subunit of F 0 F 1 ATPase was attached by a system of anti-β antibody-biotin-avidin-biotin-second antibody (specific for clenbuterol) as a biosensor to detect clenbuterol residues. The capturing mechanism was based on the antibody-antigen reaction, while the detecting mechanism depended on a fluorescence change responding to ph changes during the rotary catalytic ATP synthesis process. The results showed that the activity of F 0 F 1 -ATPase was affected with different loads and the new biosensor may prove to be a useful nano-device for super-sensitive (10 -12 g/litre clenbuterol) detection.