Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline

A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following thi...

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Published inAnalytical methods Vol. 1; no. 27; pp. 3386 - 3392
Main Authors Yu, Hui, Zhuang, Daina, Hu, Xingjuan, Zhang, Shuang, He, Zhiyong, Zeng, Maomao, Fang, Xubo, Chen, Jie, Chen, Xiaoe
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 01.01.2018
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Abstract A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100-1200 ng per spot (adjusted R -square = 0.9987), precision (relative standard deviation, 1.07-2.76%), accuracy (with a trueness of 1.76-6.22%), recovery rate (93.78-98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales. A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software.
AbstractList A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p-nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100–1200 ng per spot (adjusted R-square = 0.9987), precision (relative standard deviation, 1.07–2.76%), accuracy (with a trueness of 1.76–6.22%), recovery rate (93.78–98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales.
A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100-1200 ng per spot (adjusted R -square = 0.9987), precision (relative standard deviation, 1.07-2.76%), accuracy (with a trueness of 1.76-6.22%), recovery rate (93.78-98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales. A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software.
A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100–1200 ng per spot (adjusted R -square = 0.9987), precision (relative standard deviation, 1.07–2.76%), accuracy (with a trueness of 1.76–6.22%), recovery rate (93.78–98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales.
Author Yu, Hui
Hu, Xingjuan
Zhang, Shuang
He, Zhiyong
Zhuang, Daina
Zeng, Maomao
Fang, Xubo
Chen, Xiaoe
Chen, Jie
AuthorAffiliation Zhoushan Entry-Exit Inspection and Quarantine Bureau
State Key Laboratory of Food Science and Technology
Zhejiang Ocean University
Jiangnan University
School of Food and Pharmacy
AuthorAffiliation_xml – name: School of Food and Pharmacy
– name: Jiangnan University
– name: Zhejiang Ocean University
– name: State Key Laboratory of Food Science and Technology
– name: Zhoushan Entry-Exit Inspection and Quarantine Bureau
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Snippet A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less...
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SubjectTerms Amines
Biogenic amines
business enterprises
Chromatography
computer software
detection limit
diazonium compounds
Economic conditions
Fish
High performance liquid chromatography
Histamine
Image analysis
Image processing
Linearity
Liquid chromatography
markets
methanol
monitoring
nitroanilines
Pretreatment
Quantitation
Reagents
sales
Seafood
seafoods
standard deviation
Thin layer chromatography
Visualization
Title Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline
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https://www.proquest.com/docview/2221048723
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