Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline
A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following thi...
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Published in | Analytical methods Vol. 1; no. 27; pp. 3386 - 3392 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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Cambridge
Royal Society of Chemistry
01.01.2018
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Abstract | A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized
p
-nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100-1200 ng per spot (adjusted
R
-square = 0.9987), precision (relative standard deviation, 1.07-2.76%), accuracy (with a trueness of 1.76-6.22%), recovery rate (93.78-98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales.
A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized
p
-nitroaniline reagent, and quantitation by image analysis using the ImageJ software. |
---|---|
AbstractList | A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p-nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100–1200 ng per spot (adjusted R-square = 0.9987), precision (relative standard deviation, 1.07–2.76%), accuracy (with a trueness of 1.76–6.22%), recovery rate (93.78–98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales. A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100-1200 ng per spot (adjusted R -square = 0.9987), precision (relative standard deviation, 1.07-2.76%), accuracy (with a trueness of 1.76-6.22%), recovery rate (93.78-98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales. A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotized p -nitroaniline reagent, and quantitation by image analysis using the ImageJ software. Following this procedure, histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent's positive compounds. Moreover, this method does not require any expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100–1200 ng per spot (adjusted R -square = 0.9987), precision (relative standard deviation, 1.07–2.76%), accuracy (with a trueness of 1.76–6.22%), recovery rate (93.78–98.24%), limit of detection (23.49 ng per spot), and limit of quantitation (78.29 ng per spot). The proposed method is simpler, faster and less expensive than HPLC and the previously reported TLC/HPTLC. Therefore, this method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales. |
Author | Yu, Hui Hu, Xingjuan Zhang, Shuang He, Zhiyong Zhuang, Daina Zeng, Maomao Fang, Xubo Chen, Xiaoe Chen, Jie |
AuthorAffiliation | Zhoushan Entry-Exit Inspection and Quarantine Bureau State Key Laboratory of Food Science and Technology Zhejiang Ocean University Jiangnan University School of Food and Pharmacy |
AuthorAffiliation_xml | – name: School of Food and Pharmacy – name: Jiangnan University – name: Zhejiang Ocean University – name: State Key Laboratory of Food Science and Technology – name: Zhoushan Entry-Exit Inspection and Quarantine Bureau |
Author_xml | – sequence: 1 givenname: Hui surname: Yu fullname: Yu, Hui – sequence: 2 givenname: Daina surname: Zhuang fullname: Zhuang, Daina – sequence: 3 givenname: Xingjuan surname: Hu fullname: Hu, Xingjuan – sequence: 4 givenname: Shuang surname: Zhang fullname: Zhang, Shuang – sequence: 5 givenname: Zhiyong surname: He fullname: He, Zhiyong – sequence: 6 givenname: Maomao surname: Zeng fullname: Zeng, Maomao – sequence: 7 givenname: Xubo surname: Fang fullname: Fang, Xubo – sequence: 8 givenname: Jie surname: Chen fullname: Chen, Jie – sequence: 9 givenname: Xiaoe surname: Chen fullname: Chen, Xiaoe |
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Snippet | A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less... |
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StartPage | 3386 |
SubjectTerms | Amines Biogenic amines business enterprises Chromatography computer software detection limit diazonium compounds Economic conditions Fish High performance liquid chromatography Histamine Image analysis Image processing Linearity Liquid chromatography markets methanol monitoring nitroanilines Pretreatment Quantitation Reagents sales Seafood seafoods standard deviation Thin layer chromatography Visualization |
Title | Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline |
URI | https://www.proquest.com/docview/2068518871 https://www.proquest.com/docview/2221048723 |
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