Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation

• Published in: Applied biochemistry and biotechnology Vol. 180; no. 7; pp. 1446 - 1455
• Main Authors: Jiang, Yu, Tao, Rongsheng, Shen, Zhengquan, Sun, Liangdong, Zhu, Fuyun, Yang, Sheng
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2016
• Subjects: Acetate Kinase - metabolism; Adenosine Triphosphate - biosynthesis; Biochemistry; Biotechnology; Biotechnology - methods; Chemistry; Chemistry and Materials Science; Dipeptides - metabolism; Escherichia coli; Escherichia coli - metabolism; Glutamate-Cysteine Ligase - metabolism; Glutathione - biosynthesis; Glutathione Synthase - metabolism; Mutation - genetics; Streptococcus - enzymology; Streptococcus thermophilus; Acetate kinase; ATP generation; Bifunctional γ-glutamylcysteine synthetase/glutathione synthetase; Glutathione

Glutathione (γ-glutamyl-L-cysteinylglycine, GSH) is a pharmaceutical compound often used in food additives and the cosmetics industry. GSH can be produced biologically from L-glutamic acid, L-cysteine, and glycine through an enzymatic process traditionally involving two sequential adenosine triphosp...