Comparison between IDMS-traceable Jaffe and enzymatic creatinine assays for estimation of glomerular filtration rate by the CKD-EPI equation in healthy and diabetic subjects

• Published in: Clinical biochemistry Vol. 46; no. 15; pp. 1423 - 1429
• Main Authors: Cheuiche, Amanda Veiga, Soares, Ariana Aguiar, Camargo, Eduardo Guimarães, Weinert, Letícia Schwerz, Camargo, Joíza Lins, Silveiro, Sandra Pinho
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2013
• Subjects: Adult; Brazil; CKD-EPI; Creatinine; Creatinine - blood; Cross-Sectional Studies; Diabetes mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - physiopathology; Enzymatic; Enzyme Assays - statistics & numerical data; Female; Glomerular Filtration Rate; Humans; Jaffe; Kidney function; Male; MDRD; Middle Aged; Sensitivity and Specificity; Brazil; eGFR; Creatinine; Enzymatic; Diabetes mellitus; CKD-EPI; MDRD; Kidney function; Jaffe; Glomerular filtration rate; Modification of Diet in Renal Disease; estimated glomerular filtration rate; Chronic Kidney Disease Epidemiology Collaboration
• ISSN: 0009-9120; 1873-2933; 1873-2933
• DOI: 10.1016/j.clinbiochem.2013.05.067

The aim of this paper was to compare the agreement between creatinine measured by Jaffe and enzymatic methods and their putative influence on eGFR as calculated by the CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation in healthy and diabetic individuals. Cross-sectional study conducted in 123 adult southern Brazilians with GFR>60mL/min/1.73m2 (53 patients with type 2 diabetes, 70 healthy volunteers). Mean age was 49±16years (range of 19–86). Most were female (55%) and white (83%). Creatinine was measured by a traceable Jaffe method (Modular P, Roche Diagnostic) and by an enzymatic method (CREA plus, Roche/Hitachi 917). GFR was measured by the 51Cr-EDTA single-injection method. Serum creatinine measured by the Jaffe and enzymatic methods was similar in healthy subjects (0.79±0.16 vs. 0.79±0.15mg/dL, respectively, P=0.76), and diabetic patients (0.96±0.22 vs. 0.92±0.29mg/dL, respectively, P=0.17). However, the correlation between the two methods was higher in the healthy group (r=0.90 vs. 0.76, P<0.001). The difference between Jaffe creatinine and enzymatic creatinine was <10% in 63% of cases in the healthy group and 40% of cases in the diabetes group (P=0.018). In the subset of patients with diabetes, eGFR based on enzymatic assay results showed better agreement with measured GFR than did eGFR based on Jaffe results. Jaffe and enzymatic creatinine methods show adequate agreement in healthy subjects, but in the presence of diabetes, the enzymatic method performed slightly better. •Renal function study conducted in healthy and diabetic adults•Creatinine measured by traceable Jaffe and enzymatic methods, and GFR by 51Cr-EDTA.•Jaffe and enzymatic creatinine methods showed adequate agreement in healthy adults.•In the presence of diabetes, the enzymatic method performed slightly better.