Characterization of a human preadipocyte cell strain with high capacity for adipose differentiation

• Published in: International Journal of Obesity Vol. 25; no. 1; pp. 8 - 15
• Main Authors: Wabitsch, M, Brenner, RE, Melzner, I, Braun, M, Möller, P, Heinze, E, Debatin, K-M, Hauner, H
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 01.01.2001; Nature Publishing Group
• Subjects: Adipocytes; Adipocytes - classification; Adipocytes - metabolism; Adipose Tissue; Adipose Tissue - cytology; Adipose Tissue - growth & development; administration & dosage; agonists; anatomy and morphology; Antibiotics; Babies; Biological and medical sciences; Body fat; cell differentiation; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cell Division; cell lines; Cells; Cells, Cultured; classification; cortisol; culture media; cytology; drug effects; Female; Gene Expression; genetics; glucose; glycerol-3-phosphate dehydrogenase; Glypicans; growth & development; Growth Disorders; Growth Disorders - genetics; Growth Disorders - pathology; Heparan Sulfate Proteoglycans; Heparan Sulfate Proteoglycans - genetics; Humans; Infant; Infants; Insulin; Insulin - administration & dosage; Karyotyping; Leptin; Leptin - metabolism; lipid metabolism; lipoprotein lipase; Male; Medical sciences; messenger RNA; Metabolic diseases; Metabolism; Models, Biological; mutation; Obesity; pathology; Pediatrics; pharmacology; physiology; receptors; Receptors, Cytoplasmic and Nuclear; Receptors, Cytoplasmic and Nuclear - agonists; Receptors, Cytoplasmic and Nuclear - metabolism; Syndrome; Thiazoles; Thiazoles - pharmacology; Thiazolidinediones; Transcription Factors; Transcription Factors - agonists; Transcription Factors - metabolism; triiodothyronine; United Kingdom > UK; Germany; Human; Cell proliferation; Adipocyte; Adipose tissue; Stem cell; Preadipocyte; Cell differentiation; Metabolism; In vitro

To develop and to characterize a human preadipocyte cell strain with high capacity for adipose differentiation serving as a model for studying human adipocyte development and metabolism in vitro. Cells were derived from the stromal cells fraction of subcutaneous adipose tissue of an infant with Simp...