Agrobacterium-mediated transformation of the commercially important grapefruit cultivar Rio Red (Citrus paradisi Macf.)

• Published in: Plant cell reports Vol. 19; no. 12; pp. 1203 - 1211
• Main Authors: YANG, Z. N, INGELBRECHT, I. L, LOUZADA, E, SKARIA, M, MIRKOV, T. E
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.12.2000; Springer Nature B.V
• Subjects: agglutinins; Agrobacterium; Biological and medical sciences; Biotechnology; Citrus paradisi; Citrus tristeza virus; Cultivars; Fruits; Fundamental and applied biological sciences. Psychology; Gene expression; Genetic engineering; Genetic technics; gna gene; Methods. Procedures. Technologies; Plant tissues; Proteins; Seedlings; Shoots; Transgenic animals and transgenic plants; Transgenic plants; uidA gene; uncp gene; Monocotyledones; Insecticide; Plant pathogen; Citrus fruit; Biological control; Rutaceae; Citrus paradisi; Agrobacterium tumefaciens; Tissue culture; Microorganism resistance; Gene; Dicotyledones; Angiospermae; Bacteria; Transgenic plant; Coat protein; Closterovirus; Citrus tristeza virus; Vegetals; Genetic transformation; Pesticides; Galanthus nivalis; Gene expression; Virus; Closteroviridae; Agglutinin; Pest resistance; Spermatophyta; Rhizobiaceae; Amaryllidaceae; Cultivar; 4-MUG: 4-Methylumbelliferyl-d-glucuronic acid; NOS: Nopaline synthase; Keywords Grapefruit; GUS: β-Glucuronidase; LB: Luria broth; GNA: Galanthusnivalis agglutinin; nptII: Neomycin phosphotransferase II gene; uncp: Untranslatable coat protein gene; DBA3: Regeneration medium; gna: Galanthus nivalis agglutinin gene; MS: Murashige and Skoog; uidA: β-Glucuronidase gene; CaMV: Cauliflower mosaic virus; FMV: Figwort mosaic virus; Galanthus nivalis agglutinin; 34S promoter; Abbreviations BG: Shoot elongation medium; CTV: Citrus tristeza virus

Transgenic plants of grapefruit cv. Rio Red (Citrus paradisi Macf.) have been obtained by Agrobacterium tumefaciens-mediated gene transfer using seedling-derived epicotyl segments as explants and kanamycin as the selective agent. The transformation procedure includes a shoot elongation phase with a...