The antiangiogenic activity of Kushecarpin D, a novel flavonoid isolated from Sophora flavescens Ait
Kushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of our continuous effort to explore Chinese traditional medicinal herbs and to identify novel natural anticancer products, the antiangiogenic prope...
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Published in | Life sciences (1973) Vol. 93; no. 21; pp. 791 - 797 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
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Elsevier Inc
13.11.2013
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Abstract | Kushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of our continuous effort to explore Chinese traditional medicinal herbs and to identify novel natural anticancer products, the antiangiogenic properties of KD were examined in vitro using a human umbilical vein endothelial cell line (ECV304).
The SRB and Trypan Blue exclusion assays were used to evaluate the effect of KD on cell proliferation. The antiangiogenic activities of KD were evaluated through studies of cell migration, cell adhesion, and tube formation. DCFH-DA and DHE fluorescent assays were used to detect the reactive oxygen species (ROS) levels. Catalase activity was detected using the colorimetric ammonium molybdate method. Cell cycle and apoptosis were measured using flow cytometry and the Hoechst 33258 staining assay.
The results indicated that KD showed antiangiogenic activity via inhibitory effects on cell proliferation, cell migration, cell adhesion, and tube formation. ROS levels were down-regulated and catalase activity was up-regulated after treatment with KD. The cell cycle was arrested at the G2/M phase, while no apoptosis was observed using the Hoechst 33258 staining assay or following the flow cytometric analysis of the sub-G1 proportion.
The antiangiogenic properties of KD, in combination with its anti-proliferative effect and ability to induce cell cycle arrest without inducing apoptosis, make it a good candidate for development as antitumor agent. However, further studies are essential to elucidate its mechanism of action. |
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AbstractList | AIMSKushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of our continuous effort to explore Chinese traditional medicinal herbs and to identify novel natural anticancer products, the antiangiogenic properties of KD were examined in vitro using a human umbilical vein endothelial cell line (ECV304).MAIN METHODSThe SRB and Trypan Blue exclusion assays were used to evaluate the effect of KD on cell proliferation. The antiangiogenic activities of KD were evaluated through studies of cell migration, cell adhesion, and tube formation. DCFH-DA and DHE fluorescent assays were used to detect the reactive oxygen species (ROS) levels. Catalase activity was detected using the colorimetric ammonium molybdate method. Cell cycle and apoptosis were measured using flow cytometry and the Hoechst 33258 staining assay.KEY FINDINGSThe results indicated that KD showed antiangiogenic activity via inhibitory effects on cell proliferation, cell migration, cell adhesion, and tube formation. ROS levels were down-regulated and catalase activity was up-regulated after treatment with KD. The cell cycle was arrested at the G2/M phase, while no apoptosis was observed using the Hoechst 33258 staining assay or following the flow cytometric analysis of the sub-G1 proportion.SIGNIFICANCEThe antiangiogenic properties of KD, in combination with its anti-proliferative effect and ability to induce cell cycle arrest without inducing apoptosis, make it a good candidate for development as antitumor agent. However, further studies are essential to elucidate its mechanism of action. Kushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of our continuous effort to explore Chinese traditional medicinal herbs and to identify novel natural anticancer products, the antiangiogenic properties of KD were examined in vitro using a human umbilical vein endothelial cell line (ECV304). The SRB and Trypan Blue exclusion assays were used to evaluate the effect of KD on cell proliferation. The antiangiogenic activities of KD were evaluated through studies of cell migration, cell adhesion, and tube formation. DCFH-DA and DHE fluorescent assays were used to detect the reactive oxygen species (ROS) levels. Catalase activity was detected using the colorimetric ammonium molybdate method. Cell cycle and apoptosis were measured using flow cytometry and the Hoechst 33258 staining assay. The results indicated that KD showed antiangiogenic activity via inhibitory effects on cell proliferation, cell migration, cell adhesion, and tube formation. ROS levels were down-regulated and catalase activity was up-regulated after treatment with KD. The cell cycle was arrested at the G2/M phase, while no apoptosis was observed using the Hoechst 33258 staining assay or following the flow cytometric analysis of the sub-G1 proportion. The antiangiogenic properties of KD, in combination with its anti-proliferative effect and ability to induce cell cycle arrest without inducing apoptosis, make it a good candidate for development as antitumor agent. However, further studies are essential to elucidate its mechanism of action. AIMS: Kushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of our continuous effort to explore Chinese traditional medicinal herbs and to identify novel natural anticancer products, the antiangiogenic properties of KD were examined in vitro using a human umbilical vein endothelial cell line (ECV304). MAIN METHODS: The SRB and Trypan Blue exclusion assays were used to evaluate the effect of KD on cell proliferation. The antiangiogenic activities of KD were evaluated through studies of cell migration, cell adhesion, and tube formation. DCFH-DA and DHE fluorescent assays were used to detect the reactive oxygen species (ROS) levels. Catalase activity was detected using the colorimetric ammonium molybdate method. Cell cycle and apoptosis were measured using flow cytometry and the Hoechst 33258 staining assay. KEY FINDINGS: The results indicated that KD showed antiangiogenic activity via inhibitory effects on cell proliferation, cell migration, cell adhesion, and tube formation. ROS levels were down-regulated and catalase activity was up-regulated after treatment with KD. The cell cycle was arrested at the G2/M phase, while no apoptosis was observed using the Hoechst 33258 staining assay or following the flow cytometric analysis of the sub-G1 proportion. SIGNIFICANCE: The antiangiogenic properties of KD, in combination with its anti-proliferative effect and ability to induce cell cycle arrest without inducing apoptosis, make it a good candidate for development as antitumor agent. However, further studies are essential to elucidate its mechanism of action. |
Author | Wang, Chun-Ming Chen, He-Ping Gao, Qing-Xiang Yuan, Cheng-Shan Cao, Mei-Ai Zhang, Xiu-Li Pu, Li-Ping |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24113074$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1155/2012/373219 10.1093/jnci/82.13.1107 10.1042/CS20120212 10.1016/j.jep.2009.12.020 10.1016/j.tet.2007.10.001 10.1016/j.canlet.2012.03.008 10.1016/j.ejps.2011.09.020 10.1007/s10555-011-9310-3 10.1021/jf900340b 10.1016/j.fertnstert.2008.05.087 10.1002/jlb.47.5.440 10.1002/jcb.23257 10.1080/01635581.2011.523503 10.1007/s00280-011-1699-4 10.1096/fj.05-5128fje 10.1080/01635580802666281 10.1016/j.fitote.2011.06.002 10.1016/j.fitote.2010.01.008 10.1016/0009-8981(91)90067-M 10.1021/np2006296 10.1016/j.tetlet.2004.09.176 10.1016/j.canlet.2005.08.025 10.2174/187152009789124673 10.1021/np990051d 10.1055/s-2006-957080 10.1016/j.canlet.2006.11.016 10.3747/co.v13i1.77 10.1089/ars.2011.4489 |
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References | Zhou, Lutterodt, Cheng, Yu (bb0160) 2009; 57 Sun, Cao, Sun, Dong, Bian, Han (bb0125) 2012; 2012 Huang, Zheng (bb0040) 2006; 239 Luo, Rankin, Liu, Daddysman, Jiang, Chen (bb0075) 2009; 61 Shojaei (bb0115) 2012; 320 Nisar, Kaleem, Khan, Adhikari, Khan, Shah (bb0090) 2011; 82 Rothe, Valet (bb0100) 1990; 47 Li, Wang, Zhanga, Jia (bb0060) 2007; 63 Mahfouz, Sharma, Lackner, Aziz, Agarwal (bb0080) 2009; 92 Schneider, Pozzi (bb0110) 2011; 30 Wilkinson-Berka, Rana, Armani, Agrotis (bb0140) 2013; 124 Lam, Alex, Lam, Tsui, Yang, Lee (bb0055) 2011; 112 Goth (bb0025) 1991; 196 Kuroyanagi, Arakawa, Hirayama, Hayashi (bb0050) 1999; 62 Liang, Zhang, Shi, Liu, Shen, Tao (bb0065) 2012; 69 Wu, Wang, Cheng, Gao (bb0145) 2004; 45 Cui, Wang, Kokudo, Fang, Tang (bb0020) 2010; 4 Coso, Harrison, Harrison, Vinh, Sobey, Drummond (bb0015) 2012; 16 Ho, Ngan Hon, Chim (bb0030) 2009; 9 Wang, Yang, Wei, Jia, Zheng (bb0135) 2004; 59 Liu, Cao, Li, Shen, Yan, Yuan (bb0070) 2010; 81 Sagar, Yance, Wong (bb0105) 2006; 13 Jin, Kim, Kang, Son, Chang, Kim (bb0045) 2010; 127 Wang, Jia, Zheng (bb0130) 2007; 73 Zhang, Cao, Pu, Huang, Gao, Yuan (bb0155) 2013; 68 Zhang, Liu, Li, Luo, Liu, Dong (bb0150) 2011; 44 Albini, Dell'Eva, Vene, Ferrari, Buhler, Noonan (bb0005) 2006; 20 Chinese-Pharmacopoeia-Commission (bb0010) 2010; 1 Priyadarsini, Vinothini, Murugan, Manikandan, Nagini (bb0095) 2011; 63 Skehan, Storeng, Scudiero, Monks, McMahon, Vistica (bb0120) 1990; 82 Morley, Ferguson, Koropatnick (bb0085) 2007; 251 Huang, Wang, Wang, Yao, Han, Yuan (bb0035) 2011; 74 Liu (10.1016/j.lfs.2013.09.025_bb0070) 2010; 81 Sun (10.1016/j.lfs.2013.09.025_bb0125) 2012; 2012 Goth (10.1016/j.lfs.2013.09.025_bb0025) 1991; 196 Skehan (10.1016/j.lfs.2013.09.025_bb0120) 1990; 82 Jin (10.1016/j.lfs.2013.09.025_bb0045) 2010; 127 Kuroyanagi (10.1016/j.lfs.2013.09.025_bb0050) 1999; 62 Morley (10.1016/j.lfs.2013.09.025_bb0085) 2007; 251 Nisar (10.1016/j.lfs.2013.09.025_bb0090) 2011; 82 Priyadarsini (10.1016/j.lfs.2013.09.025_bb0095) 2011; 63 Zhang (10.1016/j.lfs.2013.09.025_bb0150) 2011; 44 Li (10.1016/j.lfs.2013.09.025_bb0060) 2007; 63 Schneider (10.1016/j.lfs.2013.09.025_bb0110) 2011; 30 Rothe (10.1016/j.lfs.2013.09.025_bb0100) 1990; 47 Sagar (10.1016/j.lfs.2013.09.025_bb0105) 2006; 13 Chinese-Pharmacopoeia-Commission (10.1016/j.lfs.2013.09.025_bb0010) 2010; 1 Shojaei (10.1016/j.lfs.2013.09.025_bb0115) 2012; 320 Wu (10.1016/j.lfs.2013.09.025_bb0145) 2004; 45 Cui (10.1016/j.lfs.2013.09.025_bb0020) 2010; 4 Coso (10.1016/j.lfs.2013.09.025_bb0015) 2012; 16 Albini (10.1016/j.lfs.2013.09.025_bb0005) 2006; 20 Mahfouz (10.1016/j.lfs.2013.09.025_bb0080) 2009; 92 Wang (10.1016/j.lfs.2013.09.025_bb0130) 2007; 73 Huang (10.1016/j.lfs.2013.09.025_bb0035) 2011; 74 Lam (10.1016/j.lfs.2013.09.025_bb0055) 2011; 112 Ho (10.1016/j.lfs.2013.09.025_bb0030) 2009; 9 Zhang (10.1016/j.lfs.2013.09.025_bb0155) 2013; 68 Wang (10.1016/j.lfs.2013.09.025_bb0135) 2004; 59 Zhou (10.1016/j.lfs.2013.09.025_bb0160) 2009; 57 Liang (10.1016/j.lfs.2013.09.025_bb0065) 2012; 69 Wilkinson-Berka (10.1016/j.lfs.2013.09.025_bb0140) 2013; 124 Luo (10.1016/j.lfs.2013.09.025_bb0075) 2009; 61 Huang (10.1016/j.lfs.2013.09.025_bb0040) 2006; 239 |
References_xml | – volume: 9 start-page: 823 year: 2009 end-page: 826 ident: bb0030 article-title: Effects of oxymatrine from Ku Shen on cancer cells publication-title: Anticancer Agents Med Chem contributor: fullname: Chim – volume: 63 start-page: 12665 year: 2007 end-page: 12670 ident: bb0060 article-title: Five new eremophilane derivatives from publication-title: Tetrahedron contributor: fullname: Jia – volume: 82 start-page: 1008 year: 2011 end-page: 1011 ident: bb0090 article-title: Molecular simulations probing Kushecarpin A as a new lipoxygenase inhibitor publication-title: Fitoterapia contributor: fullname: Shah – volume: 59 start-page: 802 year: 2004 end-page: 806 ident: bb0135 article-title: In vitro effects on proliferation, telomerase activity and apoptosis of an eremophilanoid sesquiterpene from Senecio oldhamianus maxim in cultured human tumor cell lines publication-title: Pharmazie contributor: fullname: Zheng – volume: 2012 start-page: 373219 year: 2012 ident: bb0125 article-title: Antitumor activities of kushen: literature review publication-title: Evid Based Complement Altern Med contributor: fullname: Han – volume: 196 start-page: 143 year: 1991 end-page: 151 ident: bb0025 article-title: A simple method for determination of serum catalase activity and revision of reference range publication-title: Clin Chim Acta contributor: fullname: Goth – volume: 124 start-page: 597 year: 2013 end-page: 615 ident: bb0140 article-title: Reactive oxygen species, Nox and angiotensin II in angiogenesis: implications for retinopathy publication-title: Clin Sci contributor: fullname: Agrotis – volume: 4 start-page: 39 year: 2010 end-page: 47 ident: bb0020 article-title: Traditional Chinese medicine and related active compounds against hepatitis B virus infection publication-title: Biosci Trends contributor: fullname: Tang – volume: 69 start-page: 317 year: 2012 end-page: 331 ident: bb0065 article-title: Matrine induces caspase-dependent apoptosis in human osteosarcoma cells in vitro and in vivo through the upregulation of Bax and Fas/FasL and downregulation of Bcl-2 publication-title: Cancer Chemother Pharmacol contributor: fullname: Tao – volume: 74 start-page: 2235 year: 2011 end-page: 2242 ident: bb0035 article-title: Tirucallane-type triterpenoids from publication-title: J Nat Prod contributor: fullname: Yuan – volume: 112 start-page: 3313 year: 2011 end-page: 3321 ident: bb0055 article-title: Nobiletin, a polymethoxylated flavonoid from citrus, shows anti-angiogenic activity in a zebrafish in vivo model and HUVEC in vitro model publication-title: J Cell Biochem contributor: fullname: Lee – volume: 45 start-page: 8855 year: 2004 end-page: 8858 ident: bb0145 article-title: Bieremoligularolide and eremoligularin, two novel sesquiterpenoids from publication-title: Tetrahedron Lett contributor: fullname: Gao – volume: 61 start-page: 554 year: 2009 end-page: 563 ident: bb0075 article-title: Kaempferol inhibits angiogenesis and VEGF expression through both HIF dependent and independent pathways in human ovarian cancer cells publication-title: Nutr Cancer contributor: fullname: Chen – volume: 44 start-page: 573 year: 2011 end-page: 579 ident: bb0150 article-title: Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice publication-title: Eur J Pharm Sci contributor: fullname: Dong – volume: 63 start-page: 218 year: 2011 end-page: 226 ident: bb0095 article-title: The flavonoid quercetin modulates the hallmark capabilities of hamster buccal pouch tumors publication-title: Nutr Cancer contributor: fullname: Nagini – volume: 92 start-page: 819 year: 2009 end-page: 827 ident: bb0080 article-title: Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa publication-title: Fertil Steril contributor: fullname: Agarwal – volume: 57 start-page: 4580 year: 2009 end-page: 4585 ident: bb0160 article-title: Anti-Inflammatory and antiproliferative activities of trifolirhizin, a flavonoid from Sophora flavescens roots publication-title: J Agric Food Chem contributor: fullname: Yu – volume: 68 start-page: 369 year: 2013 end-page: 375 ident: bb0155 article-title: A novel flavonoid isolated from Sophora flavescens exhibited anti-angiogenesis activity, decreased VEGF expression and caused G0/G1 cell cycle arrest in vitro publication-title: Pharmazie contributor: fullname: Yuan – volume: 82 start-page: 1107 year: 1990 end-page: 1112 ident: bb0120 article-title: New colorimetric cytotoxicity assay for anticancer-drug screening publication-title: J Natl Cancer Inst contributor: fullname: Vistica – volume: 127 start-page: 589 year: 2010 end-page: 595 ident: bb0045 article-title: Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of publication-title: J Ethnopharmacol contributor: fullname: Kim – volume: 73 start-page: 180 year: 2007 end-page: 184 ident: bb0130 article-title: The effect of 17 sesquiterpenes on cell viability and telomerase activity in the human ovarian cancer cell line HO-8910 publication-title: Planta Med contributor: fullname: Zheng – volume: 1 start-page: 188 year: 2010 end-page: 189 ident: bb0010 article-title: Kushen (Sophorae Flavescentis Radix) publication-title: The People's Republic of China Pharmacopoeia contributor: fullname: Chinese-Pharmacopoeia-Commission – volume: 47 start-page: 440 year: 1990 end-page: 448 ident: bb0100 article-title: Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′-dichlorofluorescin publication-title: J Leukoc Biol contributor: fullname: Valet – volume: 320 start-page: 130 year: 2012 end-page: 137 ident: bb0115 article-title: Anti-angiogenesis therapy in cancer: current challenges and future perspectives publication-title: Cancer Lett contributor: fullname: Shojaei – volume: 30 start-page: 277 year: 2011 end-page: 294 ident: bb0110 article-title: Cyclooxygenases and lipoxygenases in cancer publication-title: Cancer Metastasis Rev contributor: fullname: Pozzi – volume: 16 start-page: 1229 year: 2012 end-page: 1247 ident: bb0015 article-title: NADPH oxidases as regulators of tumor angiogenesis: current and emerging concepts publication-title: Antioxid Redox Signal contributor: fullname: Drummond – volume: 251 start-page: 168 year: 2007 end-page: 178 ident: bb0085 article-title: Tangeretin and nobiletin induce G1 cell cycle arrest but not apoptosis in human breast and colon cancer cells publication-title: Cancer Lett contributor: fullname: Koropatnick – volume: 62 start-page: 1595 year: 1999 end-page: 1599 ident: bb0050 article-title: Antibacterial and antiandrogen flavonoids from publication-title: J Nat Prod contributor: fullname: Hayashi – volume: 239 start-page: 271 year: 2006 end-page: 280 ident: bb0040 article-title: Rosmarinic acid inhibits angiogenesis and its mechanism of action in vitro publication-title: Cancer Lett contributor: fullname: Zheng – volume: 81 start-page: 524 year: 2010 end-page: 527 ident: bb0070 article-title: Alkaloids from publication-title: Fitoterapia contributor: fullname: Yuan – volume: 13 start-page: 14 year: 2006 end-page: 26 ident: bb0105 article-title: Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer — part 1 publication-title: Curr Oncol contributor: fullname: Wong – volume: 20 start-page: 527 year: 2006 end-page: 529 ident: bb0005 article-title: Mechanisms of the antiangiogenic activity by the hop flavonoid xanthohumol: NF-kappaB and Akt as targets publication-title: FASEB J contributor: fullname: Noonan – volume: 2012 start-page: 373219 year: 2012 ident: 10.1016/j.lfs.2013.09.025_bb0125 article-title: Antitumor activities of kushen: literature review publication-title: Evid Based Complement Altern Med doi: 10.1155/2012/373219 contributor: fullname: Sun – volume: 82 start-page: 1107 year: 1990 ident: 10.1016/j.lfs.2013.09.025_bb0120 article-title: New colorimetric cytotoxicity assay for anticancer-drug screening publication-title: J Natl Cancer Inst doi: 10.1093/jnci/82.13.1107 contributor: fullname: Skehan – volume: 124 start-page: 597 year: 2013 ident: 10.1016/j.lfs.2013.09.025_bb0140 article-title: Reactive oxygen species, Nox and angiotensin II in angiogenesis: implications for retinopathy publication-title: Clin Sci doi: 10.1042/CS20120212 contributor: fullname: Wilkinson-Berka – volume: 127 start-page: 589 year: 2010 ident: 10.1016/j.lfs.2013.09.025_bb0045 article-title: Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens publication-title: J Ethnopharmacol doi: 10.1016/j.jep.2009.12.020 contributor: fullname: Jin – volume: 63 start-page: 12665 year: 2007 ident: 10.1016/j.lfs.2013.09.025_bb0060 article-title: Five new eremophilane derivatives from Ligularia sagitta publication-title: Tetrahedron doi: 10.1016/j.tet.2007.10.001 contributor: fullname: Li – volume: 320 start-page: 130 year: 2012 ident: 10.1016/j.lfs.2013.09.025_bb0115 article-title: Anti-angiogenesis therapy in cancer: current challenges and future perspectives publication-title: Cancer Lett doi: 10.1016/j.canlet.2012.03.008 contributor: fullname: Shojaei – volume: 44 start-page: 573 year: 2011 ident: 10.1016/j.lfs.2013.09.025_bb0150 article-title: Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice publication-title: Eur J Pharm Sci doi: 10.1016/j.ejps.2011.09.020 contributor: fullname: Zhang – volume: 30 start-page: 277 year: 2011 ident: 10.1016/j.lfs.2013.09.025_bb0110 article-title: Cyclooxygenases and lipoxygenases in cancer publication-title: Cancer Metastasis Rev doi: 10.1007/s10555-011-9310-3 contributor: fullname: Schneider – volume: 59 start-page: 802 year: 2004 ident: 10.1016/j.lfs.2013.09.025_bb0135 article-title: In vitro effects on proliferation, telomerase activity and apoptosis of an eremophilanoid sesquiterpene from Senecio oldhamianus maxim in cultured human tumor cell lines publication-title: Pharmazie contributor: fullname: Wang – volume: 57 start-page: 4580 year: 2009 ident: 10.1016/j.lfs.2013.09.025_bb0160 article-title: Anti-Inflammatory and antiproliferative activities of trifolirhizin, a flavonoid from Sophora flavescens roots publication-title: J Agric Food Chem doi: 10.1021/jf900340b contributor: fullname: Zhou – volume: 92 start-page: 819 year: 2009 ident: 10.1016/j.lfs.2013.09.025_bb0080 article-title: Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa publication-title: Fertil Steril doi: 10.1016/j.fertnstert.2008.05.087 contributor: fullname: Mahfouz – volume: 47 start-page: 440 year: 1990 ident: 10.1016/j.lfs.2013.09.025_bb0100 article-title: Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′-dichlorofluorescin publication-title: J Leukoc Biol doi: 10.1002/jlb.47.5.440 contributor: fullname: Rothe – volume: 112 start-page: 3313 year: 2011 ident: 10.1016/j.lfs.2013.09.025_bb0055 article-title: Nobiletin, a polymethoxylated flavonoid from citrus, shows anti-angiogenic activity in a zebrafish in vivo model and HUVEC in vitro model publication-title: J Cell Biochem doi: 10.1002/jcb.23257 contributor: fullname: Lam – volume: 63 start-page: 218 year: 2011 ident: 10.1016/j.lfs.2013.09.025_bb0095 article-title: The flavonoid quercetin modulates the hallmark capabilities of hamster buccal pouch tumors publication-title: Nutr Cancer doi: 10.1080/01635581.2011.523503 contributor: fullname: Priyadarsini – volume: 69 start-page: 317 year: 2012 ident: 10.1016/j.lfs.2013.09.025_bb0065 article-title: Matrine induces caspase-dependent apoptosis in human osteosarcoma cells in vitro and in vivo through the upregulation of Bax and Fas/FasL and downregulation of Bcl-2 publication-title: Cancer Chemother Pharmacol doi: 10.1007/s00280-011-1699-4 contributor: fullname: Liang – volume: 20 start-page: 527 year: 2006 ident: 10.1016/j.lfs.2013.09.025_bb0005 article-title: Mechanisms of the antiangiogenic activity by the hop flavonoid xanthohumol: NF-kappaB and Akt as targets publication-title: FASEB J doi: 10.1096/fj.05-5128fje contributor: fullname: Albini – volume: 1 start-page: 188 year: 2010 ident: 10.1016/j.lfs.2013.09.025_bb0010 article-title: Kushen (Sophorae Flavescentis Radix) contributor: fullname: Chinese-Pharmacopoeia-Commission – volume: 61 start-page: 554 year: 2009 ident: 10.1016/j.lfs.2013.09.025_bb0075 article-title: Kaempferol inhibits angiogenesis and VEGF expression through both HIF dependent and independent pathways in human ovarian cancer cells publication-title: Nutr Cancer doi: 10.1080/01635580802666281 contributor: fullname: Luo – volume: 82 start-page: 1008 year: 2011 ident: 10.1016/j.lfs.2013.09.025_bb0090 article-title: Molecular simulations probing Kushecarpin A as a new lipoxygenase inhibitor publication-title: Fitoterapia doi: 10.1016/j.fitote.2011.06.002 contributor: fullname: Nisar – volume: 81 start-page: 524 year: 2010 ident: 10.1016/j.lfs.2013.09.025_bb0070 article-title: Alkaloids from Sophora flavescens Aition publication-title: Fitoterapia doi: 10.1016/j.fitote.2010.01.008 contributor: fullname: Liu – volume: 196 start-page: 143 year: 1991 ident: 10.1016/j.lfs.2013.09.025_bb0025 article-title: A simple method for determination of serum catalase activity and revision of reference range publication-title: Clin Chim Acta doi: 10.1016/0009-8981(91)90067-M contributor: fullname: Goth – volume: 74 start-page: 2235 year: 2011 ident: 10.1016/j.lfs.2013.09.025_bb0035 article-title: Tirucallane-type triterpenoids from Dysoxylum lenticellatum publication-title: J Nat Prod doi: 10.1021/np2006296 contributor: fullname: Huang – volume: 45 start-page: 8855 year: 2004 ident: 10.1016/j.lfs.2013.09.025_bb0145 article-title: Bieremoligularolide and eremoligularin, two novel sesquiterpenoids from Ligularia muliensis publication-title: Tetrahedron Lett doi: 10.1016/j.tetlet.2004.09.176 contributor: fullname: Wu – volume: 239 start-page: 271 year: 2006 ident: 10.1016/j.lfs.2013.09.025_bb0040 article-title: Rosmarinic acid inhibits angiogenesis and its mechanism of action in vitro publication-title: Cancer Lett doi: 10.1016/j.canlet.2005.08.025 contributor: fullname: Huang – volume: 68 start-page: 369 year: 2013 ident: 10.1016/j.lfs.2013.09.025_bb0155 article-title: A novel flavonoid isolated from Sophora flavescens exhibited anti-angiogenesis activity, decreased VEGF expression and caused G0/G1 cell cycle arrest in vitro publication-title: Pharmazie contributor: fullname: Zhang – volume: 9 start-page: 823 year: 2009 ident: 10.1016/j.lfs.2013.09.025_bb0030 article-title: Effects of oxymatrine from Ku Shen on cancer cells publication-title: Anticancer Agents Med Chem doi: 10.2174/187152009789124673 contributor: fullname: Ho – volume: 62 start-page: 1595 year: 1999 ident: 10.1016/j.lfs.2013.09.025_bb0050 article-title: Antibacterial and antiandrogen flavonoids from Sophora flavescens publication-title: J Nat Prod doi: 10.1021/np990051d contributor: fullname: Kuroyanagi – volume: 4 start-page: 39 year: 2010 ident: 10.1016/j.lfs.2013.09.025_bb0020 article-title: Traditional Chinese medicine and related active compounds against hepatitis B virus infection publication-title: Biosci Trends contributor: fullname: Cui – volume: 73 start-page: 180 year: 2007 ident: 10.1016/j.lfs.2013.09.025_bb0130 article-title: The effect of 17 sesquiterpenes on cell viability and telomerase activity in the human ovarian cancer cell line HO-8910 publication-title: Planta Med doi: 10.1055/s-2006-957080 contributor: fullname: Wang – volume: 251 start-page: 168 year: 2007 ident: 10.1016/j.lfs.2013.09.025_bb0085 article-title: Tangeretin and nobiletin induce G1 cell cycle arrest but not apoptosis in human breast and colon cancer cells publication-title: Cancer Lett doi: 10.1016/j.canlet.2006.11.016 contributor: fullname: Morley – volume: 13 start-page: 14 year: 2006 ident: 10.1016/j.lfs.2013.09.025_bb0105 article-title: Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer — part 1 publication-title: Curr Oncol doi: 10.3747/co.v13i1.77 contributor: fullname: Sagar – volume: 16 start-page: 1229 year: 2012 ident: 10.1016/j.lfs.2013.09.025_bb0015 article-title: NADPH oxidases as regulators of tumor angiogenesis: current and emerging concepts publication-title: Antioxid Redox Signal doi: 10.1089/ars.2011.4489 contributor: fullname: Coso |
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Snippet | Kushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of our... AIMS: Kushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part... AIMSKushecarpin D (KD) is a novel flavonoid isolated from the traditional Chinese herbal medicine Kushen (the dried root of Sophora flavescens Ait). As part of... |
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SubjectTerms | Angiogenesis Inhibitors - isolation & purification Angiogenesis Inhibitors - pharmacology Antiangiogenic apoptosis Apoptosis - drug effects Benzofurans - isolation & purification Benzofurans - pharmacology Benzopyrans - isolation & purification Benzopyrans - pharmacology catalase Catalase - metabolism cell adhesion Cell Adhesion - drug effects cell cycle Cell Cycle - drug effects Cell cycle arrest cell movement Cell Movement - drug effects cell proliferation Cell Proliferation - drug effects colorimetry Drugs, Chinese Herbal - pharmacology endothelial cells Flavonoid flavonoids Flavonoids - pharmacology flow cytometry fluorescence herbal medicines Human Umbilical Vein Endothelial Cells Humans Kushecarpin D mechanism of action medicinal plants molybdates Plant Roots - chemistry Reactive oxygen species Reactive Oxygen Species - metabolism Sophora - chemistry Sophora flavescens Sophora flavescens Ait |
Title | The antiangiogenic activity of Kushecarpin D, a novel flavonoid isolated from Sophora flavescens Ait |
URI | https://dx.doi.org/10.1016/j.lfs.2013.09.025 https://www.ncbi.nlm.nih.gov/pubmed/24113074 https://search.proquest.com/docview/1448222289 |
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