TNF-α antagonist improves oxidative stress and lipid disorders induced by scorpion venom in the intestinal tissue

• Published in: Acta tropica Vol. 185; pp. 307 - 313
• Main Authors: Saidoune-Malek, Imane, Ait-Lounis, Aouatef, Laraba-Djebari, Fatima
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2018
• Subjects: Aah venom; Animals; Intestinal Mucosa - metabolism; Intestine tissue; Intestines - drug effects; Lipid metabolism; Lipid Metabolism - drug effects; Lipids - analysis; Mice; Mice, Inbred Strains; Oxidative stress; Oxidative Stress - drug effects; Scorpion Venoms - toxicity; TNF-α antagonist; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - physiology; Intestine tissue; Oxidative stress; Lipid metabolism; Aah venom; TNF-α antagonist
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2018.06.013

[Display omitted] •TNF-α antagonist improves intestinal oxidative damage induced by Aah venom.•TNF-α antagonist improves intestinal cytotoxicity in Aah-envenomed mice.•TNF-α antagonist improves postprandial serum lipid in Aah-envenomed mice.•TNF-α antagonist improves intestinal lipid profile in Aah-injected mice. We previously reported that Androctonus australis hector (Aah) venom induces inflammation in several tissues, however limited information is available on its role in gastrointestinal tract. Here we evaluate the involvement of TNF-α in lipid metabolism in the small intestine after Aah envenomation. To address these issues, NMRI mice (3-month-old) were pre-treated with a TNF-α antagonist, 30 min prior to Aah venom injection. Redox status, cytotoxicity and histopathological changes were analyzed in small intestine 3 and 24 h after Aah injection. Lipid metabolism was evaluated through lipid tolerance test in sera. Lipid content in small intestine was also evaluated at different times after envenomation. Obtained results showed that Aah venom affects the intestinal integrity. This cytotoxicity could be associated with lipid peroxidation and altered or insufficient antioxidant system. These results also highlight the perturbation of lipid absorption in intestine tissue of envenomed mice. The use of TNF-α antagonist prior to Aah venom injection seems to be able to improve lipid profile, oxidative stress and antioxidant activity. These findings suggest that Aah venom induces lipid alterations in the intestinal tissue mechanisms involving of TNF- α.