UHPLC-ESI-MS/MS assay for quantification of endocannabinoids in cerebrospinal fluid using surrogate calibrant and surrogate matrix approaches

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 222; p. 115090
• Main Authors: Aydin, Ece, Cebo, Malgorzata, Mielnik, Justyna, Richter, Hardy, Schüle, Rebecca, Sievers-Engler, Adrian, Młynarz, Piotr, Lämmerhofer, Michael
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.01.2023
• Subjects: 2-arachidonoylglycerol; Anandamide; Cerebrospinal fluid; Endocannabinoid; Surrogate matrix; UHPLC-MS/MS; MeCN; SPE; Cerebrospinal fluid; SLE; SRM; 2-AGE; QC; Surrogate matrix; SPME; Endocannabinoid; CSF; ESI; AEA; LOD; MM; LEA; IS; OEA; PEA; 2-arachidonoylglycerol; LOQ; Anandamide; CB2; CB1; UHPLC-MS/MS; 1- and 2-AG; MeOH; aCSF

Endocannabinoids are endogenous lipids with the main function recognized to act as neuromodulators through their cannabinoid receptors. Dysregulation of the endocannabinoid system is implicated in various pathologies, such as inflammatory and neurodegenerative diseases. In this study we describe a s...