Production of recombinant adeno-associated virus vectors using a packaging cell line and a hybrid recombinant adenovirus

• Published in: Gene therapy Vol. 6; no. 2; pp. 293 - 299
• Main Authors: LIU, X. L, CLARK, K. R, JOHNSON, P. R
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 01.02.1999
• Subjects: Adenoviridae - genetics; Adenoviruses; Biological and medical sciences; Biotechnology; Cell Line; Dependovirus - genetics; DNA, Recombinant; Expression vectors; Fundamental and applied biological sciences. Psychology; Gene therapy; Genetic Therapy - methods; Genetic Vectors; Genomes; Health. Pharmaceutical industry; Humans; Industrial applications and implications. Economical aspects; Packaging; Plasmids; Transfection; Viruses; Virus; Human; Associated virus; Adenoviridae; Cell line; Recombinant virus; Production; Gene therapy; Vector

Recombinant adeno-associated virus (rAAV) vectors are under consideration for a wide variety of gene therapy applications. One of the limitations of the rAAV vector system has been the difficulty in producing the vector in sufficient quantity for adequate preclinical and clinical evaluation. A commo...