CRISPRi screens identify the lncRNA, LOUP , as a multifunctional locus regulating macrophage differentiation and inflammatory signaling

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 121; no. 22; p. e2322524121
• Main Authors: Halasz, Haley, Malekos, Eric, Covarrubias, Sergio, Yitiz, Samira, Montano, Christy, Sudek, Lisa, Katzman, Sol, Liu, S John, Horlbeck, Max A, Namvar, Leila, Weissman, Jonathan S, Carpenter, Susan
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28.05.2024
• Subjects: Biological Sciences; Cell Differentiation - genetics; CRISPR-Cas Systems; Differentiation; Gene Expression Regulation; Genes; High-throughput screening; Humans; Immune system; Inflammation; Inflammation - genetics; Inflammation - metabolism; Innate immunity; Macrophages; Macrophages - cytology; Macrophages - metabolism; Monocytes; Monocytes - cytology; Monocytes - metabolism; NF-kappa B - metabolism; NF-κB protein; Non-coding RNA; Open reading frames; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; PU.1 protein; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Signal Transduction; TLR4 protein; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Trans-Activators - genetics; Trans-Activators - metabolism; Transcriptomes; long noncoding RNA; macrophage; CRISPRi; inflammation; short encoded peptide

Long noncoding RNAs (lncRNAs) account for the largest portion of RNA from the transcriptome, yet most of their functions remain unknown. Here, we performed two independent high-throughput CRISPRi screens to understand the role of lncRNAs in monocyte function and differentiation. The first was a repo...