Analysis of the Protective Effects of Rosa roxburghii -Fermented Juice on Lipopolysaccharide-Induced Acute Lung Injury in Mice through Network Pharmacology and Metabolomics

• Published in: Nutrients Vol. 16; no. 9; p. 1376
• Main Authors: Chen, Zhiyu, Zhang, Shuo, Sun, Xiaodong, Meng, Duo, Lai, Chencen, Zhang, Min, Wang, Pengjiao, Huang, Xuncai, Gao, Xiuli
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.04.2024
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - drug therapy; Acute Lung Injury - metabolism; Acute Lung Injury - prevention & control; Animals; Cytokines - metabolism; Disease Models, Animal; Drug dosages; Energy Metabolism - drug effects; Fermentation; Fruit and Vegetable Juices; Lipopolysaccharides; Lung - drug effects; Lung - metabolism; Lung - pathology; Male; Medical research; Metabolism; Metabolites; metabolomics; Metabolomics - methods; Mice; molecular docking; Molecular Docking Simulation; Network Pharmacology; Oxidative Stress - drug effects; Pharmacology; Plant Extracts - pharmacology; Reagents; Rosa - chemistry; Rosa roxburghii-fermented juice; Software; United States > US; China; molecular docking; metabolomics; network pharmacology; Rosa roxburghii-fermented juice
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16091376

Acute lung injury, a fatal condition characterized by a high mortality rate, necessitates urgent exploration of treatment modalities. Utilizing UHPLS-Q-Exactive Orbitrap/MS, our study scrutinized the active constituents present in -fermented juice (RRFJ) while also assessing its protective efficacy against LPS-induced ALI in mice through lung histopathological analysis, cytokine profiling, and oxidative stress assessment. The protective mechanism of RRFJ against ALI in mice was elucidated utilizing metabolomics, network pharmacology, and molecular docking methodologies. Our experimental findings demonstrate that RRFJ markedly ameliorates pathological injuries in ALI-afflicted mice, mitigates systemic inflammation and oxidative stress, enhances energy metabolism, and restores dysregulated amino acid and arachidonic acid metabolic pathways. This study indicates that RRFJ can serve as a functional food for adjuvant treatment of ALI.