Observations Regarding On-Column, Flow-Induced Degradation During SEC Analysis

• Published in: Journal of liquid chromatography & related technologies Vol. 31; no. 20; pp. 3105 - 3114
• Main Author: Striegel, André M.
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 03.11.2008; Taylor & Francis
• Subjects: Analytical chemistry; Applied sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Crosslinking and degradation; Exact sciences and technology; Extensional flow; On-column degradation; Organic polymers; Other chromatographic methods; Physicochemistry of polymers; Polymers and radiations; Properties and characterization; Size-exclusion chromatography; Structure, morphology and analysis; Ultrasonication; Depolymerization; Extensional flow; Sonication; Ultrasonication; Flow rate; Refractometry; Styrene polymer; On-column degradation; Size-exclusion chromatography; Comparative study; Gel permeation chromatography; Operating conditions

The flow-induced degradation of ultra-high molar mass (M) polymers during their passage through a size-exclusion chromatography (SEC) column has been a subject of interest for several decades. Studies in this regard have been relatively few, however. Of interest is knowing whether degradation occurs...