Substrate promiscuity of pyruvate kinase on various deoxynucleoside diphosphates for synthesis of deoxynucleoside triphosphates

• Published in: Enzyme and microbial technology Vol. 43; no. 6; pp. 455 - 459
• Main Authors: Gao, Shuhong, Bao, Jie, Gu, Xiaoming, Xin, Xiujuan, Chen, Changhua, Ryu, Dewey D.Y.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 06.11.2008; Elsevier Science
• Subjects: Bacillus; Bacillus sp . PK; Biological and medical sciences; Biotechnology; Deoxynucleoside triphosphate (dNTP); Escherichia coli; Fundamental and applied biological sciences. Psychology; Promiscuity; Pyruvate kinase (PK); Zymomonas mobilis; Zymomonasmobilis PK; Zymomonas mobilis PK; Deoxynucleoside triphosphate (dNTP); Bacillus sp . PK; Pyruvate kinase (PK); Promiscuity; Pyruvate kinase; Enzyme; Transferases; Bacillus sp. PK; Bacillaceae; Substrate; Bacillales; Bacillus; Bacteria; Zymomonas mobilis

Directed regulation of the promiscuity of pyruvate kinase (PK) on different deoxynucleoside diphosphate (dNDP) substrates is an effective way to improve the process efficiency of biosynthesis of deoxynucleoside triphosphates (dNTP). The rabbit muscle PK was found to be highly specific on adenosine d...