Isolation of Mycobacterium lepromatosis and Development of Molecular Diagnostic Assays to Distinguish Mycobacterium leprae and M. lepromatosis

• Published in: Clinical infectious diseases Vol. 71; no. 8; pp. e262 - e269
• Main Authors: Sharma, Rahul, Singh, Pushpendra, McCoy, Rajiv C, Lenz, Shannon M, Donovan, Kelly, Ochoa, Maria T, Estrada-Garcia, Iris, Silva-Miranda, Mayra, Jurado-Santa Cruz, Fermin, Balagon, Marivic F, Stryjewska, Barbara, Scollard, David M, Pena, Maria T, Lahiri, Ramanuj, Williams, Diana L, Truman, Richard W, Adams, Linda B
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 05.11.2020
• Subjects: Animals; Humans; Mexico; Mice; Mycobacterium; Mycobacterium leprae - genetics; Online Only; Pathology, Molecular; Mexico; leprosy diagnostic assay; real-time PCR; Mycobacterium leprae; Mycobacterium lepromatosis

Abstract Background Mycobacterium leprae was thought to be the exclusive causative agent of leprosy until Mycobacterium lepromatosis was identified in a rare form of leprosy known as diffuse lepromatous leprosy (DLL). Methods We isolated M. lepromatosis from a patient with DLL and propagated it in a...