A practicable method to prepare nitrated proteins with peroxynitrite and low concentration of sodium hydroxide

• Published in: Molecular biology reports Vol. 47; no. 2; pp. 1393 - 1398
• Main Authors: Zhang, Nan, Gao, Xiaochen, Zhang, Weijia, Higazy, Doaa, Wang, Ke, Fu, Zhenfang, Cui, Min
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2020; Springer Nature B.V
• Subjects: Aging; Animal Anatomy; Animal Biochemistry; Animals; Biochemistry - methods; Biological activity; Biomedical and Life Sciences; Caustic soda; Chemokine CCL2 - metabolism; CHO Cells; Cricetinae; Cricetulus; Histology; Hydrochloric acid; Life Sciences; Methods Paper; Morphology; Nitrates - metabolism; Nitration; Oxidants; Peroxynitrite; Peroxynitrous Acid - metabolism; Proteins; Proteins - metabolism; Sodium; Sodium hydroxide; Sodium Hydroxide - pharmacology; Sodium nitrite; Tryptophan; Tyrosine; CCL2; Peroxynitrite; Method; Protein nitration; Free radical; Syntheses

Peroxynitrite is an ion acting as a powerful oxidant and nucleophile, which plays a key role in the inflammation and aging process by nitrating tyrosine or tryptophan residues of the proteins. Nitration of a target protein is considered to be a proper method to study the behavioral change of the pro...