Neophytadiene from Turbinaria ornata Suppresses LPS-Induced Inflammatory Response in RAW 264.7 Macrophages and Sprague Dawley Rats

• Published in: Inflammation Vol. 43; no. 3; pp. 937 - 950
• Main Authors: Bhardwaj, Meenakshi, Sali, Veeresh Kumar, Mani, Sugumar, Vasanthi, Hannah R.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2020; Springer Nature B.V
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Algae; Animals; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antioxidants; Biological Factors - isolation & purification; Biological Factors - pharmacology; Biological Factors - therapeutic use; Biomedical and Life Sciences; Biomedicine; Cytokines; Dose-Response Relationship, Drug; Enzyme-linked immunosorbent assay; Immunology; Inflammation; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - metabolism; Inflammation Mediators - antagonists & inhibitors; Inflammation Mediators - metabolism; Interleukin 1; Interleukin 10; Interleukin 6; Internal Medicine; Lipopolysaccharides; Lipopolysaccharides - toxicity; Macrophages; Macrophages - drug effects; Macrophages - metabolism; Male; MAP kinase; Mice; mRNA; NF-κB protein; Nitric oxide; Nitric-oxide synthase; Original Article; Pathology; Phaeophyceae; Pharmacology/Toxicology; Random Allocation; Rats; Rats, Sprague-Dawley; RAW 264.7 Cells; Rheumatology; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Tumors; Turbinaria ornata; NF-κB; Neophytadiene; lipopolysaccharide; inflammation; TLR4

This study investigates the mode of action of Neophytadiene (MT), a molecule isolated from a marine algae Turbinaria ornata in LPS-induced inflammation in both in vitro and in vivo conditions. Neophytadiene (25, 50, 100 μM/mL) was treated to LPS-stimulated RAW 264.7 macrophages cells to identify its...