The effect of zinc aspartate pretreatment on ischemia-reperfusion injury and early changes of blood and tissue antioxidant enzyme activities after unilateral testicular torsion-detorsion

• Published in: Journal of pediatric surgery Vol. 39; no. 1; pp. 91 - 95
• Main Authors: ÖZKAN, Y. K. U, BORAN, C, KILINC, M, GARIPARDIC, M, KURUTAS, E. B
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Elsevier Inc 2004; Elsevier
• Subjects: Animals; Antioxidants - metabolism; Aspartic Acid - analogs & derivatives; Aspartic Acid - pharmacology; Aspartic Acid - therapeutic use; Biological and medical sciences; Catalase - blood; Catalase - metabolism; Disease Models, Animal; General aspects; Gynecology. Andrology. Obstetrics; ischemia-reperfusion; Male; Male genital diseases; Malondialdehyde - blood; Malondialdehyde - metabolism; Medical sciences; Non tumoral diseases; Rats; Rats, Sprague-Dawley; Reperfusion Injury - enzymology; Reperfusion Injury - etiology; Reperfusion Injury - prevention & control; Spermatic Cord Torsion - complications; Spermatic Cord Torsion - drug therapy; Spermatic Cord Torsion - enzymology; Superoxide Dismutase - blood; Superoxide Dismutase - metabolism; testicular torsion; Testis - drug effects; Testis - enzymology; Testis - pathology; Zinc aspartate; Zinc aspartate; testicular torsion; ischemia-reperfusion; Pediatrics; Nervous system diseases; Injury; Cardiovascular disease; Aspartate; Change; Antioxidant; Blood; Zinc; Vascular disease; Medicine; Spermatic cord diseases; Tissue; Reperfusion; Enzymatic activity; Ischemia; Excitatory aminoacid; Neurotransmitter; Unilateral; Spermatic cord torsion; Early; Lesion; Pretreatment; Male genital diseases
• ISSN: 0022-3468; 1531-5037
• DOI: 10.1016/j.jpedsurg.2003.09.013

The aim of this study is to investigate the effect of zinc aspartate (ZA) pretreatment on ischemia-reperfusion (I/R) injury and blood and tissue antioxidant enzyme activity early after unilateral testicular torsion-detorsion (T/D). Forty prepubertal male Sprague-Dawley rats (weight 160 to 220 g) were divided into 4 groups each containing 10 rats. Surgery was conducted under intraperitoneal 1-shot ketamine (50 mg/kg) anesthesia. The scrotum was entered through a midline incision. Rotating the left testis 720° in a clockwise direction was the model of the testicular torsion. Group 1 was for the basal values. Group 2 had 4 hours T/D. Group 3 also had 4 hours T/D and pretreated with 50 mg/kg intraperitoneal ZA injection half an hour before detorsion. Group 4 was designed as a sham group. In the Group 2 and Group 3, the tunica vaginalis was opened, and left testicles were rotated clockwise 720° and maintained in this torsion position by fixing with a silk suture to the scrotal wall. The scrotum was closed and 4 hours later reentered for testicular detorsion. After spermatic cord detorsion, the scrotum was closed. At the end of 4 hours detorsion period, bilateral orchiectomies were performed, and 5-mL intracardiac blood samples were taken. Blood and tissue superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde (MDA) levels were measured, and histopathologic examination was performed. Group 2 and group 3 had decreased blood SOD and CAT activities and elevated MDA levels indicating I/R injury. The 2 groups were also different from each other for these parameters reflecting the beneficial effect of ZA pretreatment ( P < .05). The decreased ipsilateral tissue SOD and CAT activities in group 2 were different from the other groups including group 3 ( P < .05). Ipsilateral tissue MDA levels of both group 2 and group 3 were elevated. Group 2 had higher values than group 3 ( P < .05). In addition, specimens from group 2 had a significantly greater histologic injury than group 3 ( P < .05). These findings were also supporting the beneficial effect of ZA pretreatment. All measurements of contralateral tests were similar to the basal values for all groups ( P > .05). ZA pretreatment reduces I/R injury by its antioxidant effects after unilateral testicular T/D and affects the antioxidant enzyme systems.