The involvement of genes related to bile secretion pathway in rat tooth germ development

Tooth formation is accomplished under strict genetic control procedures. Therefore, exploring the gene network system of tooth development has a very positive practical significance for the study of tooth tissue regeneration and the prevention and treatment of tooth abnormalities. Early bell stage i...

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Published inJournal of molecular histology Vol. 51; no. 1; pp. 99 - 107
Main Authors Yang, Jun, Lu, Xi, Liu, Shangfeng, Zhao, Shouliang
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.02.2020
Springer Nature B.V
Subjects
Ameloblasts
Aquaporin 4
Bile
Biomedical and Life Sciences
Biomedicine
Cell Biology
Dental enamel
Developmental Biology
Developmental stages
Embryos
Epithelium
Genes
Genetic control
Life Sciences
Odontoblasts
Original Paper
Ribonucleic acid
RNA
Teeth
Bile secretion pathway
Odontogenesis
Tooth germ
RNA-seq
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ISSN1567-2379
1567-2387
1567-2387
DOI10.1007/s10735-020-09861-0

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Summary:Tooth formation is accomplished under strict genetic control procedures. Therefore, exploring the gene network system of tooth development has a very positive practical significance for the study of tooth tissue regeneration and the prevention and treatment of tooth abnormalities. Early bell stage is the initial phase of odontoblast formation and dentin matrix deposition in the process of tooth development. Through RNA sequencing and differential gene analysis of the rat tooth germ samples at cap stage and early bell stage, we found that the bile secretion pathway was the most significant difference signal pathway during the development between cap stage and bell stage, which mainly included ABCC3, AQP4, SLC10A1, SLC2A1, SLC4A4, ADCY5, AQP9, CFTR, ATP1A2, ATP1B1 and ATP1A1, totally 11genes. Immunostaining revealed that SLC2A1, SLC4A4, ADCY5 and ATP1B1were mainly expressed in epithelium in bud stage and inner and outer enamel epithelium during the embryonic phase. In the postnatal 1 and postnatal 7, SLC2A1, SLC4A4 and ABCC3 were highly expressed in ameloblasts and odontoblasts while ADCY5, ATP1B1 and SLC10A1was expressed moderately only in odontoblasts. This finding illustrated that the bile secretion pathway related genes may participate in the development of tooth germ.
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ISSN:1567-2379
1567-2387
1567-2387
DOI:10.1007/s10735-020-09861-0