Single-cell transcriptomic data reveal the increase in extracellular matrix organization and antigen presentation abilities of fibroblasts and smooth muscle cells in patients with pelvic organ prolapse

• Published in: International Urogynecology Journal Vol. 34; no. 10; pp. 2529 - 2537
• Main Authors: Fan, Weimin, Wu, Duanqing, Zhang, Liwen, Ye, Jun, Guan, Junhua, Yang, Ying, Mei, Xiaohui, Chen, Rujun
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2023; Springer Nature B.V
• Subjects: Antigen presentation; Extracellular matrix; Fibroblasts; Gynecology; Ligands; Medicine; Medicine & Public Health; Original Article; Pelvic organ prolapse; Smooth muscle; Urology; Vagina; Smooth muscle cell; Antigen presentation; Pelvic organ prolapse; Fibroblast; Extracellular matrix organization
• DOI: 10.1007/s00192-023-05539-9
• ISSN: 0937-3462; 1433-3023

Introduction and hypothesis We aimed to explore the cellular properties of fibroblasts and smooth muscle cells (SMCs), the two major cell types of the vagina wall, in pelvic organ prolapse (POP) to improve the knowledge of the underlying molecular mechanisms of POP. Methods The single-cell RNA sequencing (scRNA-seq) profile GSE151202 was downloaded from NCBI Gene Expression Omnibus, in which vaginal wall tissues were harvested from patients with anterior vaginal wall prolapse and control subjects respectively. The scRNA-seq data of samples (5 POP and 5 controls) were adopted for analysis. Cluster analysis was performed to identify the cell subclusters. Trajectory analysis was applied to construct the differentiation trajectories of fibroblasts and SMCs. Cellular communication analysis was carried out to explore the ligand–receptor interactions between fibroblasts/SMCs and immune cells. Results Ten subclusters were determined in both groups, among which fibroblasts and SMCs were the most abundant cell types. Compared with controls, fibroblasts increased whereas SMCs declined in POP. During the transition of fibroblasts and SMCs from a normal into a disease state, extracellular matrix organization and antigen presentation were heightened. The intercellular communications were altered in POP. Interactions between fibroblasts/SMCs and macrophages/natural killer/T cells were strengthened as more ligand–receptor pairs involved in antigen presentation pathways were gained in POP. Conclusion Extracellular matrix organization and antigen presentation abilities of fibroblasts and SMCs were enhanced in POP.