Placental supernatants’ enhancement of the metastatic potential of breast cancer cells: is estrogen receptor (ERα) essential for this phenomenon?

• Published in: Archives of gynecology and obstetrics Vol. 300; no. 4; pp. 981 - 991
• Main Authors: Bar, Michal, Komemi, Oded, Pomeranz, Meir, Fishman, Ami, Drucker, Liat, Lishner, Michael, Tartakover Matalon, Shelly
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2019; Springer Nature B.V
• Subjects: Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell adhesion & migration; Cell Cycle; Cell Movement; Cell Proliferation; Endocrinology; Estrogen Receptor alpha - genetics; Estrogen Receptor alpha - metabolism; Estrogen Receptor alpha - physiology; Estrogens - metabolism; Female; Gynecologic Oncology; Gynecology; Human Genetics; Humans; MCF-7 Cells; Medicine; Medicine & Public Health; Obstetrics/Perinatology/Midwifery; Placenta - chemistry; Placenta - metabolism; Pregnancy; Pregnancy Complications, Neoplastic - pathology; Pregnancy Trimester, First; Progesterone - metabolism; STAT3 Transcription Factor - metabolism; Womens health; Pregnancy; Breast cancer; Placenta; ERα; STAT3
• ISSN: 0932-0067; 1432-0711
• DOI: 10.1007/s00404-019-05243-4

Purpose Pregnancy-associated breast cancer (PABC) is usually diagnosed at an advanced stage in comparison to non-pregnant women. The placenta secretes hormones and cytokines, which affect breast cancer progression. Previously, we demonstrated that human placental secretome facilitates the survival and migration of ERα+ breast cancer cells (BCCL), but pregnant women have a relatively high frequency of ERα-negative tumors. In the current study, we analyzed the effect of placental secretome on ERα-negative BCCL. Methods BCCL [MCF-7(estrogen/progesterone receptor positive (ERα+/PR+), ERα reduced MCF-7 (siRNA, MCF-7 ERα−), HS-578 and BT-549 cells (both ER−/PR−)] were exposed to supernatants (collected from first trimester human placental explants and from control BCCL) or to E2 + P4 (estrogen + progesterone) in placental supernatant concentrations and then tested for cell proliferation (number, cell cycle, PCNA), cell-death, cell migration, STAT3 pathway activation and functionality. Results Silencing ERα in the MCF-7 cells negated the placental supernatant and E2 + P4 enhancement of cell migration (> 130%, p  < 0.05), number (> 120%) and survival (~ 130%). However, it had no such effect on MCF-7-ER− migration, which was still elevated in the presence of placental secretome. ER−/PR− BCCL were unaffected by the hormones, but placental secretome significantly elevated their migration (115%), number (140–170%), STAT3 phosphorylation (~ 180%) and BT-549 STAT3 level. These effects were negated by the STAT3 inhibitor. Conclusions Placental supernatant facilitates BCCL malignant characteristics by activating ERα in estrogen responsive cells and STAT3 in ERα- BCCL. This indicates a possible mechanism that may underlie PABC’s advanced state and suggests STAT3 pathway as a therapeutic target for PABC.