Nrf2 Mediates the Anti-apoptotic and Anti-inflammatory Effects Induced by Gastrodin in Hydrogen Peroxide–Treated SH-SY5Y Cells

• Published in: Journal of molecular neuroscience Vol. 69; no. 1; pp. 115 - 122
• Main Authors: de Oliveira, Marcos Roberto, Brasil, Flávia Bittencourt, Fürstenau, Cristina Ribas
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.09.2019; Springer Nature B.V
• Subjects: Adenosine diphosphate; Anti-inflammatory agents; Apoptosis; Bcl-2 protein; Benzyl Alcohols - pharmacology; Biomedical and Life Sciences; Biomedicine; Brain; Caspase; Caspase-3; Caspase-9; Cell Biology; Cell death; Cell fate; Cell Line, Tumor; Cytochrome; Cytochrome c; Cytochromes; Cytosol; Deoxyribonucleic acid; DNA; DNA Fragmentation; Glucosides - pharmacology; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; IL-1β; Inflammation; Interleukin-1beta - metabolism; Interleukins; Natural products; Neurochemistry; Neurodegeneration; Neurology; Neurons - drug effects; Neurons - metabolism; Neuroprotective Agents - pharmacology; Neurosciences; NF-E2-Related Factor 2 - metabolism; NF-kappa B - metabolism; NRF2 protein; Poly(ADP-ribose) polymerase; Poly(ADP-ribose) Polymerases - metabolism; Proteomics; Ribose; siRNA; Transcription activation; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; NF-κB; Anti-apoptotic; Nrf2; Anti-inflammatory; Gastrodin
• DOI: 10.1007/s12031-019-01339-3
• ISSN: 0895-8696

Redox impairment, inflammation, and increased rates of cell death are central players during neurodegeneration. In that context, activation of the transcription factor nuclear factor erythroid 2–related factor 2 (Nrf2) has been viewed as an interesting strategy in order to reduce the impact of redox dysfunction and neuroinflammation on cell fate. There is evidence indicating that the benefits caused by natural products in the brain may be due to the ability of these agents in upregulating Nrf2. Gastrodin (GAS) induces anti-oxidant, anti-inflammatory, and anti-apoptotic actions in brain cells. Nonetheless, the mechanisms underlying such effects are not clear yet. Therefore, we investigated here whether GAS would affect apoptosis and inflammation in the human neuroblastoma cell line (SH-SY5Y) exposed to hydrogen peroxide (H 2 O 2 ). GAS at 1–25 μM was administrated to the cells during 30 min before a challenge with H 2 O 2 at 300 μM for additional 24 h. GAS prevented the activation of the intrinsic apoptotic pathway by modulating the levels of Bcl-2 and Bax, causing a decrease in the release of cytochrome c to the cytosol. GAS also prevented the activation of the pro-apoptotic enzymes caspase-9 and caspase-3. Consequently, GAS abrogated poly (ADP-ribose) polymerase (PARP) cleavage and DNA fragmentation in the H 2 O 2 -treated SH-SY5Y cells. Moreover, GAS reduced the levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) and the activity of nuclear factor-κB in H 2 O 2 -treated cells. Silencing of Nrf2 by small interfering RNA (siRNA) suppressed the GAS-induced cytoprotection. Thus, GAS elicited anti-apoptotic and anti-inflammatory effects by a mechanism involving Nrf2 in SH-SY5Y cells.