Bacterial community characterization of Batura Glacier in the Karakoram Range of Pakistan

• Published in: International microbiology Vol. 24; no. 2; pp. 183 - 196
• Main Authors: Ali, Pervaiz, Chen, Feng, Hassan, Fariha, Sosa, Ana, Khan, Samiullah, Badshah, Malik, Shah, Aamer Ali
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.05.2021; Spanish Society for Microbiology
• Subjects: Actinobacteria; Antiinfectives and antibacterials; Antimicrobial activity; Applied Microbiology; Arthrobacter; Bacteria; Bacteroidetes; Biomedical and Life Sciences; Cell culture; Colistin; Colistin sulfate; DNA sequencing; Drug resistance; Eukaryotic Microbiology; Flavobacterium; Glaciers; Gram-positive bacteria; High altitude; Klebsiella; Life Sciences; Medical Microbiology; Metabolites; Methicillin; Microbial Ecology; Microbiology; Microorganisms; Multidrug resistance; Nalidixic acid; Original Article; Phylogenetics; Phylogeny; Proteobacteria; rRNA 16S; Sediments; Strains (organisms); Sulfamethoxazole; Sulfate resistance; Trimethoprim; Vancomycin; Pakistan; Cold-adapted bacteria; Psychrotrophs; Metagenomics; Batura Glacier; Karakoram
• ISSN: 1139-6709; 1618-1905
• DOI: 10.1007/s10123-020-00153-x

High-altitude cold habitats of the Karakoram are rarely explored for their bacterial community characterization and metabolite productions. In the present study, bacterial communities in ice, water, and sediments of Batura Glacier were investigated using culture-dependent and culture-independent methods. Twenty-seven cold-adapted bacterial strains (mostly psychrotrophic) were isolated using R2A, Tryptic Soy Agar (TSA), and Luria-Bertani (LB) media, at 4 °C and 15 °C. Most of the isolates exhibited growth at a wide range of temperature (4–35 °C), pH (5–12), and salinity (1–6%). Among the bacterial isolates, 52% were identified as Gram-positive and the remaining 48% represented as Gram-negative. The results of phylogenetic analysis indicated that all the culturable bacteria belonged to 3 major phylogenetic groups, i.e., Actinobacteria (48%), Bacteroidetes (26%), and Proteobacteria (22%), while Flavobacterium (26%), Arthrobacter (22%), and Pseudomonas (19%) were represented as the dominant genera. Similarly, Illumina amplicon sequencing of 16S rRNA genes after PCR amplification of DNA from the whole community revealed dominance of the same phylogenetic groups, Proteobacteria, Actinobacteria, and Bacteroidetes, while Arthrobacter , Mycoplana , Ochrobactrum , Kaistobacter , Janthinobacterium , and Flavobacterium were found as the dominant genera. Among the culturable isolates, 70% demonstrated activity for cellulases, 48% lipases, 41% proteases, 41% DNases, and only 7% for amylases. Most of the glacial isolates demonstrated antimicrobial activity against other microorganisms including the multiple-drug-resistant strains of Candida albicans , Klebsiella pneumoniae , Acinetobacter sp., and Bacillus sp. 67% of Gram-negative while 46% of Gram-positive glacial bacteria were resistant to trimethoprim/sulfamethoxazole. Resistance against methicillin and vancomycin among the Gram-positive isolates was 23% and 15%, respectively, while 11% of the Gram-negative isolates exhibited resistance against both colistin sulfate and nalidixic acid.