Quality assessment on the long-term cryopreservation and nucleic acids extraction processes implemented in the andalusian public biobank

• Published in: Cell and tissue banking Vol. 20; no. 2; pp. 255 - 265
• Main Authors: Ortega-Pinazo, J., Díaz, T., Martínez, B., Jiménez, A., Pinto-Medel, M. J., Ferro, P.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2019; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Blood Banks; Cell Biology; Cell Survival; Cell viability; Citric acid; Cryopreservation; Cryopreservation - methods; Deoxyribonucleic acid; DNA; DNA - analysis; Freezing; Full Length Paper; Humans; Leukocytes (mononuclear); Leukocytes, Mononuclear - cytology; Life Sciences; Nucleic acids; Peripheral blood mononuclear cells; Quality control; Ribonucleic acid; RNA; RNA - analysis; Spain; Statistical analysis; Tissue Banks; Transplant Surgery; Spain; Whole blood; RNA; Cryopreservation; DNA; Biobank; PBMCs; PCR

Human samples are commonly collected and long-term stored in biobanks for current and future analyses. Even though techniques for freezing human blood are well established, the storage time can compromise the cell viability as well as the yield and quality of nucleic acids (RNA and DNA) extracted fr...