SLC7A9 as a Potential Biomarker for Lymph Node Metastasis of Esophageal Squamous Cell Carcinoma
Background The expression of solute carrier (SLC) 7 family genes is reportedly associated with several malignancies. Here, we focused on SLC7A9 and investigated its expression, function, and clinical significance in esophageal squamous cell carcinoma (ESCC). Methods SLC7A9 transcription levels were...
Saved in:
Published in | Annals of surgical oncology Vol. 29; no. 4; pp. 2699 - 2709 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.04.2022
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Background
The expression of solute carrier (SLC) 7 family genes is reportedly associated with several malignancies. Here, we focused on
SLC7A9
and investigated its expression, function, and clinical significance in esophageal squamous cell carcinoma (ESCC).
Methods
SLC7A9
transcription levels were evaluated in 13 ESCC cell lines, and polymerase chain reaction (PCR) array analysis was conducted to detect coordinately expressed genes with
SLC7A9
.
SLC7A9
contributions to proliferation, invasion, and migration were evaluated in ESCC cells subjected to siRNA-mediated gene knockdown and pCMV6-entry plasmid-mediated overexpression.
SLC7A9
expression was detected in 189 ESCC tissues by quantitative reverse-transcription (qRT)-PCR and correlated with clinicopathological parameters.
Results
The expression levels of
SLC7A9
varied widely in ESCC cell lines and correlated with
FGFBP1
expression. Knockdown of
SLC7A9
significantly suppressed the proliferation, invasion, and migration of the ESCC cell lines. Moreover, overexpression of
SLC7A9
enhanced cell proliferation and migration. In analyses of clinical specimens,
SLC7A9
mRNA was overexpressed in the ESCC tissues compared with the adjacent normal esophageal tissues. High mRNA expression was significantly associated with high levels of squamous cell carcinoma-related antigen and carcinoembryonic antigen, advanced disease stage, and lymph node metastasis. High
SLC7A9
expression was also significantly associated with poor disease-specific and disease-free survival, and lymph node recurrence after radical surgery, but not with the other recurrence patterns. On multivariate analysis, high
SLC7A9
expression was an independent predictor of lymph node recurrence.
Conclusions
SLC7A9
influences the malignant behavior of ESCC cells. Tumor
SLC7A9
expression may serve as a novel biomarker for predicting lymph node metastasis and recurrence in ESCC patients. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1068-9265 1534-4681 |
DOI: | 10.1245/s10434-021-11001-1 |