Heat stress aggravates oxidative stress, apoptosis, and endoplasmic reticulum stress in the cerebellum of male C57 mice

• Published in: Molecular biology reports Vol. 48; no. 8; pp. 5881 - 5887
• Main Authors: Oghbaei, Hajar, Hosseini, Leila, Farajdokht, Fereshteh, Rahigh Aghsan, Sepideh, Majdi, Alireza, Sadigh-Eteghad, Saeed, Sandoghchian Shotorbani, Siamak, Mahmoudi, Javad
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2021; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Apoptosis; Apoptosis - physiology; Biomedical and Life Sciences; Brain - metabolism; Caspase-3; Cerebellum; Cerebellum - metabolism; Cerebellum - physiology; Endoplasmic reticulum; Endoplasmic Reticulum - pathology; Endoplasmic Reticulum Stress - physiology; Heat shock proteins; Heat stress; Heat-Shock Proteins - metabolism; Heat-Shock Response - genetics; Heat-Shock Response - physiology; Histology; Hsp70 protein; Life Sciences; Male; Mice; Mice, Inbred C57BL; Morphology; Original Article; Oxidative stress; Oxidative Stress - physiology; Reactive oxygen species; Reactive Oxygen Species - metabolism; Cerebellum; Oxidative stress; Heat stress; Heat shock protein (HSP)70; Apoptosis; Endoplasmic reticulum stress
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06582-9

Background The current study was set to assess the effect of heat stress exposure on oxidative stress, apoptosis, and endoplasmic reticulum stress markers in the cerebellum of male mice. Methods Fifty male C57BL/6 mice were assigned to five groups of (I) control, (II) heat stress (HS)7, (III) HS14, (IV) HS21, and (V) HS42 groups. Animals in the control group were not exposed to HS. Mice in the II-V groups were exposed to HS once a day over 7, 14, 21, and 42 days, respectively. Cerebellar reactive oxygen species (ROS) levels, expression of heat shock protein (HSP)70 and caspase 3 as well as endoplasmic reticulum stress-related proteins (PERK, p-PERK, CHOP, and Full-length ATF-6) expression were determined on the 7th, 14th, 21st, and 42nd days. Results ROS levels and HSP70 expression increased following HS on the 14th, 21st, and 42nd days and the 7th, and 14th days with a peak level of expression on the 14th day following HS. HSP70 levels decreased afterward on the 21st and 42nd days compared with the control group. Besides, exposure to HS for 14, 21, and 42 days resulted in a significant increase in the CHOP and p-PERK levels in the cerebellum compared with the control group. Heat exposure also increased protein expression of cleaved caspase 3 and active ATF-6/Full–length ATF-6 on the 21st and 42nd days in the cerebellum compared with the control animals. Conclusion These findings indicated that chronic HS augmented oxidative stress, endoplasmic reticulum stress, and apoptosis pathways in the cerebellum of mice.