Hymenobacter persicinus sp. nov., a novel member of the family Hymenobacteraceae

• Published in: Antonie van Leeuwenhoek Vol. 112; no. 7; pp. 1019 - 1028
• Main Authors: Ten, Leonid N., Jeon, Na Yeong, Li, Weilan, Kim, Myung Kyum, Cho, Young-Je, Jung, Hee-Young
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.07.2019; Springer Nature B.V
• Subjects: Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Base Composition; Biomedical and Life Sciences; Cytophagaceae - classification; Cytophagaceae - genetics; Cytophagaceae - isolation & purification; Cytophagaceae - metabolism; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Fatty Acids - metabolism; Gene sequencing; Hybridization; Hymenobacter; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Original Paper; Phosphatidylethanolamine; Phylogeny; Plant Sciences; Quinones; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sodium chloride; Soil Science & Conservation; Republic of Korea; Soil bacteria; Polyphasic taxonomy; Hymenobacteraceae; Hymenobacter
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-019-01236-0

A bacterial strain, 1-3-3-3 T , was isolated from a soil sample collected in Jeollabuk-do province, South Korea. Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be red-pink in colour. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Hymenobacter in the family Hymenobacteraceae , with high levels of 16S rRNA sequence similarity with Hymenobacter algoricola VUG-A23a T (98.0%), Hymenobacter knuensis 16F7C-2 (97.9%), Hymenobacter fastidiosus VUG-A124 T (97.1%), Hymenobacter elongatus VUG-A112 T (97.0%), Hymenobacter chitinivorans Txc1 T (97.0%) and Hymenobacter aquaticus 16F3P T (96.7%). Growth of strain 1-3-3-3 T was observed at 10–30 °C, pH 6–8 and in the presence of 0–1.0% NaCl. The genomic G + C content was determined to be 61.6 mol %. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as iso-C 15:0 (19.9%), summed feature 3 (C 16:1 ω 7 c /C 16:1 ω 6 c , 19.7%), summed feature 4 (iso-C 17:1 I/anteiso-C 17:1 B, 17.8%), C 16:1 ω 5c (12.5%) and anteiso-C 15:0 (11.2%), and the major polar lipid was found to be phosphatidylethanolamine. The phenotypic and chemotaxonomic data support the affiliation of strain 1-3-3-3 T with the genus Hymenobacter. However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 34%. The DNA–DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 1-3-3-3 T represents a novel species in the genus Hymenobacter , for which the name Hymenobacter persicinus sp. nov. is proposed. The type strain is 1-3-3-3 T (= KCTC 52742 T  = JCM 32191 T ).