Effect of steaming process on the structural characteristics and antioxidant activities of polysaccharides from Polygonatum sibiricum rhizomes

• Published in: Glycoconjugate journal Vol. 38; no. 5; pp. 561 - 572
• Main Authors: Li, Qinying, Zeng, Jun, Gong, Pixian, Wu, Yanchao, Li, Huijing
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2021; Springer Nature B.V
• Subjects: Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Benzothiazoles - chemistry; Biochemistry; Biomedical and Life Sciences; Biphenyl Compounds - chemistry; Drying; Food processing; Fourier transforms; Functional foods & nutraceuticals; Hydroxyl Radical - chemistry; Infrared spectroscopy; Life Sciences; Monosaccharides; Original Article; Pathology; Picrates - chemistry; Polygonatum; Polygonatum - chemistry; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Rhizome - chemistry; Rhizomes; Spectrum analysis; Sulfonic Acids - chemistry; Characterization; Radical scavenging; rhizomes; Steaming; Polysaccharide; Polygonatum sibiricum rhizomes
• DOI: 10.1007/s10719-021-10013-z
• ISSN: 0282-0080; 1573-4986

Polygonatum sibiricum ( P. sibiricum ) rhizomes are widely used as a tonic and functional food, and are often processed to enhance their tonic function by repeated steaming and drying. As the most important constituent, the polysaccharide from P. sibiricum rhizomes (PSP) has demonstrated various activities, but the alteration of structural characteristics and activities of the purified PSPs during steaming process was rarely investigated. To well understand the effect of steaming process on the polysaccharides of P. sibiricum , neutral polysaccharides from P. sibiricum rhizomes (PSP0 ~ PSP9) after steaming were first isolated and purified, and then the chemical properties and antioxidant activities were determined. The results showed that the molar ratios of monosaccharides in PSPs were different. The molecular weights of PSPs were increased significantly after the fourth steaming. Morphological studies showed that the surface of PSPs became much tighter during the steaming process. Fourier transform infrared spectroscopy spectra displayed the polysaccharides had similar backbones and chemical groups. Furthermore, the antioxidant activity of PSPs was measured through radical scavenging tests. It was found that the radical scavenging activity of PSPs was elevated strikingly after steaming, and increased gradually with numbers of steaming process. The biological and chemical variance of PSPs revealed considerable segregation of PSP0, PSP1 ~ PSP4 and PSP5 ~ PSP9. In conclusion, our results proposed the fourth time as the optimal number of steaming to extract functional polysaccharide from P. sibiricum rhizomes.