Evaluation of ASPM and TEF Gene Expressions as Potential Biomarkers for Bladder Cancer

• Published in: Biochemical genetics Vol. 58; no. 3; pp. 490 - 507
• Main Authors: Saleh, Amany A., Gohar, Suzy F., Hemida, Aiat Shaban, Elgharbawy, Mohamed, Soliman, Shimaa E.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2020; Springer Nature B.V
• Subjects: Adult; Aged; Basic-Leucine Zipper Transcription Factors - genetics; Biochemistry; Biomarkers; Biomarkers, Tumor - genetics; Biomedical and Life Sciences; Biomedicine; Bladder; Bladder cancer; Cancer; Disease Progression; Down-Regulation - genetics; Female; Follow-Up Studies; Gene Expression; Gene Expression Regulation, Neoplastic; Genes; Genitourinary tract; Human Genetics; Humans; Kaplan-Meier Estimate; Male; Medical Microbiology; Medical prognosis; Metastases; Middle Aged; Nerve Tissue Proteins - genetics; Original Article; Prognosis; Progression-Free Survival; Sensitivity; Survival; Survival analysis; Tef gene; Tissues; Tumorigenesis; Tumors; Up-Regulation - genetics; Urinary bladder; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - mortality; Urinary Bladder Neoplasms - pathology; Zoology; ASPM; Metastasis; TEF; Real-time PCR; Bladder cancer
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1007/s10528-020-09962-1

Bladder cancer is one of the most predominant tumors of the genitourinary tract. In addition to pathological findings, the molecular modifications that might affect tumorigenesis and tumor outcome should be considered when treating bladder cancer. Accordingly, we aimed to investigate the expression levels of both the ASPM and TEF genes in bladder cancer tissues and their value in disease prognosis. The expression levels of the ASPM and TEF genes were analyzed by quantitative real-time PCR (qRT-PCR) in 90 bladder cancer tissue specimens and 90 specimens of normal urinary bladder tissue taken away from the tumor site. The upregulation of ASPM expression and the downregulation of TEF expression were observed in bladder cancer tissues compared to adjacent normal tissues, and these levels were correlated with high-grade tumors, advanced stage disease and the presence of metastasis. Both genes had the ability to predict metastatic association with sensitivity (84.62%) and specificity (68.42%; *P < 0.001) for the ASPM gene and for the TEF gene with sensitivity (80.77%) and specificity (78.95%; *P < 0.001). Additionally, Kaplan–Meier survival analysis indicated that elevated ASPM expression levels and reduced TEF expression levels significantly correlated with decreased overall survival and progression-free survival. The current analysis concludes that ASPM and TEF expressions might be used as potential biomarkers in bladder cancer patients.