Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme

• Published in: The American journal of physiology Vol. 249; no. 1 Pt 1; p. E107
• Main Authors: Iverius, P H, Brunzell, J D
• Format: Journal Article
• Language: English
• Published: United States 01.07.1985
• Subjects: Adipose Tissue - enzymology; Adolescent; Adult; Aged; Detergents; Eating; Female; Heparin; Humans; Immunochemistry; Lipoprotein Lipase - blood; Lipoprotein Lipase - isolation & purification; Lipoprotein Lipase - metabolism; Middle Aged; Obesity - enzymology; Reference Values; Temperature

An assay procedure using three different methods to recover lipoprotein lipase (LPL) activity from biopsy specimens of human adipose tissue has been developed. Elution of enzyme from small pieces of tissue was performed at 4 and 37 degrees C using a physiological buffer containing heparin and serum....