Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study

• Published in: Lasers in medical science Vol. 35; no. 9; pp. 1909 - 1918
• Main Authors: Oliveira Prado Bergamo, Mariel Tavares, Vitor, Luciana Lourenço Ribeiro, Lopes, Nathalia Martins, Lourenço Neto, Natalino, Dionísio, Thiago José, Oliveira, Rodrigo Cardoso, Oliveira Rodini, Camila, Santos, Carlos Ferreira, Machado, Maria Aparecida Andrade Moreira, Oliveira, Thais Marchini
• Format: Journal Article
• Language: English
• Published: London Springer London 01.12.2020; Springer Nature B.V
• Subjects: Angiogenesis; CD31 antigen; Cell viability; Dentistry; Dosimeters; Dosimetry; Enzyme-linked immunosorbent assay; Irradiation; Lasers; Light therapy; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Photonics; Platelet-derived growth factor; Protein biosynthesis; Protein expression; Protein synthesis; Proteins; Quantum Optics; Semiconductor lasers; Stem cells; Vascular endothelial growth factor; Photobiomodulation therapy; Angiogenic proteins; Stem cell; Dental pulp
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-020-02975-7

This study evaluated the viability, proliferation, and protein expression after photobiomodulation (PBM) of stem cell from human exfoliated deciduous teeth (SHED). The groups were the following: G1 (2.5 J/cm2), G2 (3.7 J/cm2), and control (not irradiated). According to the groups, cells were irradiated with InGaAlP diode laser at 660 nm wavelength, continuous mode, and single time application. After 6 h, 12 h, and 24 h from irradiation, the cell viability and proliferation, and the protein expression were analyzed by MTT, crystal violet, and ELISA multiplex assay, respectively. Twenty-four hours after PBM, SHED showed better proliferation. Over time in the supernatant, all groups had an increase at the levels of VEGF-C, VEGF-A, and PLGF. In the lysate, the control and G2 exhibited a decrease of the VEGF-A, PECAM-1, and PLGF expression, while control and G3 decreased VEGF-C, VEGF-A, and PDGF expression. The dosimetries of 2.5 J/cm2 and 3.7 J/cm2 maintained viability, improved proliferation, and synthesis of the angiogenic proteins in the supernatant in the studied periods on SHED.