A simple, rapid and sensitive HILIC LC-MS/MS method for simultaneous determination of 16 purine metabolites in plasma and urine

• Published in: Talanta (Oxford) Vol. 267; p. 125171
• Main Authors: Li, Xiaona, Liu, Zhini, Li, Zhuo, Xiong, Xin, Zhang, Xianhua, Yang, Changqing, Zhao, Libo, Zhao, Rongsheng
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.01.2024
• Subjects: Acute kidney injury; AKI; chemical species; derivatization; freeze-thaw cycles; HILIC; humans; hydrophilicity; kidneys; LC-MS/MS; metabolism; metabolites; nucleobases; nucleosides; pathogenesis; Purine metabolites; quantitative analysis; urine; eGFR; LLOQ; Scr; AMP; Acute kidney injury; ROC analysis; IP; IS; GMP; cAMP; IMP; AUC; LC-MS/MS; QC; XMP; Purine metabolites; HQC; HILIC; HPLC; MQC; RP; ULOQ; AKI; LQC

Purine intermediates play important roles in physiological function and participate in the kidney disorders, while a targeted quantification of the metabolic alterations in the purine metabolism in acute kidney injury (AKI) individuals has not been conducted. In the study, a novel, rapid and sensiti...