Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins

• Published in: Glycoconjugate journal Vol. 40; no. 1; pp. 69 - 84
• Main Authors: Torres-Arteaga, Iovanna, Blanco-Labra, Alejandro, Mendiola-Olaya, Elizabeth, García-Gasca, Teresa, Aguirre-Mancilla, Cesar, Ortega-de-Santiago, Alondra L., Barboza, Mariana, Lebrilla, Carlito B., Castro-Guillén, José Luis
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2023; Springer Nature B.V
• Subjects: Adenocarcinoma; Affinity; Affinity chromatography; Agglutination; Amino acid sequence; Asparagine; Beans; Biochemistry; Biological activity; Biomedical and Life Sciences; Carbohydrate composition; Colonic Neoplasms; Concanavalin A; Erythrocytes; Glycosylation; Homology; Humans; Isoforms; Lectins; Lectins - chemistry; Life Sciences; Magnesium; Mannose; Mass spectroscopy; Molecular Docking Simulation; N-glycans; Original Article; Pathology; Phaseolus - chemistry; Phaseolus - metabolism; Phaseolus acutifolius; Plant Lectins - metabolism; Polysaccharides; Purification; Seeds; glycosylation; Anticancer lectins; Cancer-associated glycans; Homology modelling; Molecular docking; Tepary bean; In silico glycosylation

We present the purification and characterization of the two most abundant isoforms of lectins isolated from Tepary bean ( Phaseolu s acutifolius ) seeds, which have been shown to differentially affect the survival of different cancer cells. They were separated by concanavalin A-affinity chromatograp...