Pyrene remediation by Trametes maxima: an insight into secretome response and degradation pathway

• Published in: Environmental science and pollution research international Vol. 29; no. 29; pp. 44135 - 44147
• Main Authors: Imam, Arfin, Suman, Sunil Kumar, Vempatapu, Bhanu Prasad, Tripathi, Deependra, Ray, Anjan, Kanaujia, Pankaj K.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2022; Springer Nature B.V
• Subjects: 1-Hydroxypyrene; Acetic acid; Aquatic environment; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biodegradation; Biodegradation, Environmental; Bioremediation; Detoxification; Diethyl phthalate; Earth and Environmental Science; Economic development; Ecotoxicology; Environment; Environmental Chemistry; Environmental degradation; Environmental Health; Environmental science; Gas chromatography; Heat shock proteins; Metabolites; Microbial degradation; Microorganisms; n-Butyl phthalate; Phthalates; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; Polyporaceae; Proteins; Pyrene; Pyrenes - metabolism; Research Article; Secretome; Spectrometry; Stress proteins; Trametes - metabolism; Trametes maxima; Waste Water Technology; Water Management; Water Pollution Control; White rot; White rot fungi; Biodegradation; Pyrene; Secretome; White-rot fungi; Ligninolytic enzyme; Pyrene metabolite
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-022-18888-7

The rapid pace of economic development has resulted in the release of several polycyclic aromatic hydrocarbons (PAHs) into the environment. Microbial degradation using white-rot fungi is a promising method for the removal of PAHs from the environment. In the present study, biodegradation of recalcitrant PAH by a white-rot fungus,  Trametes maxima  IIPLC-32, was investigated using pyrene. The pyrene concentration decreased by 79.80%, 65.37%, and 56.37% within 16 days from the initial levels of 10 mg L −1 , 25 mg L −1 , and 50 mg L −1 , respectively. Gas chromatographic–mass spectrometric identification of prominent metabolites 1-hydroxypyrene, 2-methyl-1-naphthyl acetic acid, di-n-butyl phthalate, and diethyl phthalate helped in determining the pyrene degradation pathway. The presence of 81 extracellular proteins was revealed by secretome analysis. The identified proteins up-regulated in response to pyrene degradation were classified into detoxification proteins (6.12%), redox proteins (6.12%), stress proteins (4.08%), metabolic-related proteins (26.53%), translation and transcriptional proteins (49%), catalytic proteins (49%), and other proteins (8.16%). Knowledge of secretome analysis in pyrene degradation helped to understand the degradation mechanism of pyrene. Also, the study suggests that T. maxima IIPLC-32 has the potential to be used in the bioremediation of PAH contaminated aquatic environment. Graphical abstract