Uncoupling glucose sensing from GAL metabolism for heterologous lactose fermentation in Saccharomyces cerevisiae
Objectives Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in Saccharomyces cerevisiae by deleting two negative reg...
Saved in:
Published in | Biotechnology letters Vol. 43; no. 8; pp. 1607 - 1616 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.08.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Objectives
Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in
Saccharomyces cerevisiae
by deleting two negative regulatory genes,
GAL80
and
MIG1,
and introducing the essential lactose hydrolase
LAC4
and lactose transporter
LAC12
, from the native but inefficient lactose fermenting yeast
Kluyveromyces marxianus
.
Results
Previously, integration of the
LAC4
and
LAC12
genes into the
MIG1
and
NTH1
loci was achieved to construct strain AY-51024M. Low rates of lactose conversion led us to generate the
Δmig1Δgal80
diploid mutant strain AY-GM from AY-5, which exhibited loss of diauxic growth and glucose repression, subsequently taking up galactose for consumption at a significantly higher rate and yielding higher ethanol concentrations than strain AY-51024M. Similarly, in cheese whey permeate powder solution (CWPS) during three, repeated, batch processes in a 5L bioreactor containing either 100 g/L or 150 g/L lactose, the lactose uptake and ethanol productivity rates were both significantly greater than that of AY-51024M, while the overall fermentation times were considerably lower.
Conclusions
Using the Cre-loxp system for deletion of the
MIG1
and
GAL80
genes to relieve glucose repression, and
LAC4
and
LAC12
overexpression to increase lactose uptake and conversion provides an efficient basis for yeast fermentation of whey permeate by-product into ethanol. |
---|---|
AbstractList | OBJECTIVESDevelopment of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in Saccharomyces cerevisiae by deleting two negative regulatory genes, GAL80 and MIG1, and introducing the essential lactose hydrolase LAC4 and lactose transporter LAC12, from the native but inefficient lactose fermenting yeast Kluyveromyces marxianus. RESULTSPreviously, integration of the LAC4 and LAC12 genes into the MIG1 and NTH1 loci was achieved to construct strain AY-51024M. Low rates of lactose conversion led us to generate the Δmig1Δgal80 diploid mutant strain AY-GM from AY-5, which exhibited loss of diauxic growth and glucose repression, subsequently taking up galactose for consumption at a significantly higher rate and yielding higher ethanol concentrations than strain AY-51024M. Similarly, in cheese whey permeate powder solution (CWPS) during three, repeated, batch processes in a 5L bioreactor containing either 100 g/L or 150 g/L lactose, the lactose uptake and ethanol productivity rates were both significantly greater than that of AY-51024M, while the overall fermentation times were considerably lower. CONCLUSIONSUsing the Cre-loxp system for deletion of the MIG1 and GAL80 genes to relieve glucose repression, and LAC4 and LAC12 overexpression to increase lactose uptake and conversion provides an efficient basis for yeast fermentation of whey permeate by-product into ethanol. Objectives Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in Saccharomyces cerevisiae by deleting two negative regulatory genes, GAL80 and MIG1, and introducing the essential lactose hydrolase LAC4 and lactose transporter LAC12 , from the native but inefficient lactose fermenting yeast Kluyveromyces marxianus . Results Previously, integration of the LAC4 and LAC12 genes into the MIG1 and NTH1 loci was achieved to construct strain AY-51024M. Low rates of lactose conversion led us to generate the Δmig1Δgal80 diploid mutant strain AY-GM from AY-5, which exhibited loss of diauxic growth and glucose repression, subsequently taking up galactose for consumption at a significantly higher rate and yielding higher ethanol concentrations than strain AY-51024M. Similarly, in cheese whey permeate powder solution (CWPS) during three, repeated, batch processes in a 5L bioreactor containing either 100 g/L or 150 g/L lactose, the lactose uptake and ethanol productivity rates were both significantly greater than that of AY-51024M, while the overall fermentation times were considerably lower. Conclusions Using the Cre-loxp system for deletion of the MIG1 and GAL80 genes to relieve glucose repression, and LAC4 and LAC12 overexpression to increase lactose uptake and conversion provides an efficient basis for yeast fermentation of whey permeate by-product into ethanol. Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in Saccharomyces cerevisiae by deleting two negative regulatory genes, GAL80 and MIG1, and introducing the essential lactose hydrolase LAC4 and lactose transporter LAC12, from the native but inefficient lactose fermenting yeast Kluyveromyces marxianus. Previously, integration of the LAC4 and LAC12 genes into the MIG1 and NTH1 loci was achieved to construct strain AY-51024M. Low rates of lactose conversion led us to generate the Δmig1Δgal80 diploid mutant strain AY-GM from AY-5, which exhibited loss of diauxic growth and glucose repression, subsequently taking up galactose for consumption at a significantly higher rate and yielding higher ethanol concentrations than strain AY-51024M. Similarly, in cheese whey permeate powder solution (CWPS) during three, repeated, batch processes in a 5L bioreactor containing either 100 g/L or 150 g/L lactose, the lactose uptake and ethanol productivity rates were both significantly greater than that of AY-51024M, while the overall fermentation times were considerably lower. Using the Cre-loxp system for deletion of the MIG1 and GAL80 genes to relieve glucose repression, and LAC4 and LAC12 overexpression to increase lactose uptake and conversion provides an efficient basis for yeast fermentation of whey permeate by-product into ethanol. |
Author | Guo, Xuewu Zhou, Lisha Chen, Xiaohui Zou, Jing Xiao, Dongguang Chang, Xuedong Hu, Yinghong |
Author_xml | – sequence: 1 givenname: Jing orcidid: 0000-0003-2788-4187 surname: Zou fullname: Zou, Jing email: zoujing520315@126.com organization: Chestnut Engineering Research Center of Hebei Province; Chestnut Industry Collaborative Innovation Center of Hebei Province, College of Food Science and Technology, Hebei Normal University of Science and Technology – sequence: 2 givenname: Xiaohui surname: Chen fullname: Chen, Xiaohui organization: Chestnut Engineering Research Center of Hebei Province; Chestnut Industry Collaborative Innovation Center of Hebei Province, College of Food Science and Technology, Hebei Normal University of Science and Technology – sequence: 3 givenname: Yinghong surname: Hu fullname: Hu, Yinghong organization: Chestnut Engineering Research Center of Hebei Province; Chestnut Industry Collaborative Innovation Center of Hebei Province, College of Food Science and Technology, Hebei Normal University of Science and Technology – sequence: 4 givenname: Dongguang surname: Xiao fullname: Xiao, Dongguang organization: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory; College of Biotechnology, Tianjin University of Science and Technology – sequence: 5 givenname: Xuewu surname: Guo fullname: Guo, Xuewu organization: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory; College of Biotechnology, Tianjin University of Science and Technology – sequence: 6 givenname: Xuedong surname: Chang fullname: Chang, Xuedong organization: Chestnut Engineering Research Center of Hebei Province; Chestnut Industry Collaborative Innovation Center of Hebei Province, College of Food Science and Technology, Hebei Normal University of Science and Technology – sequence: 7 givenname: Lisha surname: Zhou fullname: Zhou, Lisha organization: Key Laboratory of Computer Virtual Technology and System Integration, Hebei Province; College of Information Science and Engineering, Yanshan University |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33937967$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kT1vFDEQhi0URC6BP0CBLNHQLHj8uS6jKASkkyggteX1jS8b7dqHvYuUf4-PCyBRUFkjP_PMjN4LcpZyQkJeA3sPjJkPFZjitmMcOiZA6K5_RjagjOi0MfqMbBhI6JS0_Jxc1PrAGLOGmRfkXAgrjNVmQw53KeT1MI1pT_fTGnJFWjHVYx1Lnunt1ZbOuPghT2OdacyF3uOCJU95n9dKJx-WY1PEMmNa_DLmRMdEv_oQ7n0zPAasNGDBH2MdPb4kz6OfKr56ei_J3cebb9efuu2X28_XV9suCKOWDkTPd7oH0DjYoLz0GuLOGjBG-naWFDyYAaL2JnAredBx8L3cgQpaq2jEJXl38h5K_r5iXdw81oDT5BO2vR1XHKRVou8b-vYf9CGvJbXtGiV7xq1VtlH8RIWSay0Y3aGMsy-PDpg75uFOebiWh_uVhzuq3zyp12HG3Z-W3wE0QJyA2r7SHsvf2f_R_gQZvZiG |
CitedBy_id | crossref_primary_10_1016_j_biteb_2022_101131 crossref_primary_10_1016_j_biortech_2022_127463 crossref_primary_10_1016_j_scitotenv_2021_152253 crossref_primary_10_3390_jof8040395 crossref_primary_10_1007_s10529_024_03502_2 crossref_primary_10_2139_ssrn_4076933 crossref_primary_10_1016_j_engmic_2023_100103 |
Cites_doi | 10.1007/s10295-012-1227-5 10.1002/(SICI)1097-0061(19980630)14:9<827::AID-YEA281>3.0.CO;2-N 10.1007/s10529-005-3910-3 10.1016/j.jbiotec.2007.05.027 10.1128/AEM.65.5.2020-2024.1999 10.1007/s11157-016-9402-1 10.1016/j.biotechadv.2010.02.002 10.1023/A:1001806012566 10.1093/nar/30.6.e23 10.1016/j.jbiotec.2016.04.041 10.1021/jf100602j 10.1016/j.procbio.2017.07.013 10.1002/bit.22988 10.1007/s10295-016-1821-z 10.1007/s002530051441 10.1139/w02-032 10.1038/82400 10.1007/s10295-010-0748-z 10.1074/jbc.272.48.30350 10.1128/AEM.00186-08 10.1016/j.biotechadv.2015.07.002 10.1002/j.1460-2075.1991.tb04901.x 10.1073/pnas.1418058112 10.1128/AEM.71.11.6465-6472.2005 10.1016/S1937-6448(08)01003-4 10.1126/science.223.4637.701 10.1007/s12010-016-2078-8 |
ContentType | Journal Article |
Copyright | The Author(s), under exclusive licence to Springer Nature B.V. 2021 The Author(s), under exclusive licence to Springer Nature B.V. 2021. |
Copyright_xml | – notice: The Author(s), under exclusive licence to Springer Nature B.V. 2021 – notice: The Author(s), under exclusive licence to Springer Nature B.V. 2021. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7QL 7QR 7T7 7TB 7U5 7X7 7XB 88A 88E 88I 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. L6V L7M LK8 M0S M1P M2P M7N M7P M7S P64 PQEST PQQKQ PQUKI PRINS PTHSS Q9U 7X8 |
DOI | 10.1007/s10529-021-03136-8 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Mechanical & Transportation Engineering Abstracts Solid State and Superconductivity Abstracts ProQuest Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Database (Proquest) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Engineering Collection Advanced Technologies Database with Aerospace Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) ProQuest Science Journals Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database ProQuest Engineering Database Biotechnology and BioEngineering Abstracts ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection ProQuest Central Basic MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef ProQuest Central Student ProQuest Central Essentials SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management Health Research Premium Collection Natural Science Collection Biological Science Collection Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Medical Library (Alumni) Engineering Collection Engineering Database ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Solid State and Superconductivity Abstracts Engineering Research Database ProQuest One Academic Technology Collection Technology Research Database Mechanical & Transportation Engineering Abstracts ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest Engineering Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Advanced Technologies Database with Aerospace ProQuest Central Basic ProQuest Science Journals ProQuest SciTech Collection ProQuest Medical Library Materials Science & Engineering Collection ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic ProQuest Central Student MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering Biology |
EISSN | 1573-6776 |
EndPage | 1616 |
ExternalDocumentID | 10_1007_s10529_021_03136_8 33937967 |
Genre | Journal Article |
GrantInformation_xml | – fundername: Heibei Province Key Research and Development Projects grantid: 19227119D – fundername: Doctoral Scientific Research Foundation of Hebei Normal University of Science and Technology grantid: 2013YB015 |
GroupedDBID | --- -4W -56 -5G -BR -EM -Y2 -~C .86 .VR 06C 06D 0R~ 0VY 199 1N0 1SB 2.D 203 23N 28- 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 36B 3EH 3SX 3V. 4.4 406 408 409 40D 40E 53G 5GY 5QI 5VS 67N 67Z 6J9 6NX 78A 7X7 88A 88E 88I 8AO 8FE 8FG 8FH 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X A8Z AAAVM AABHQ AABYN AAFGU AAHNG AAIAL AAJKR AANXM AANZL AAPBV AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABBBX ABBXA ABDZT ABECU ABELW ABFGW ABFTV ABHLI ABHQN ABJCF ABJNI ABJOX ABKAS ABKCH ABKTR ABMNI ABMQK ABNWP ABPLI ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFO ACGFS ACGOD ACHSB ACHXU ACIGE ACIHN ACIPQ ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPRK ACREN ACTTH ACVWB ACWMK ADBBV ADHHG ADHIR ADIMF ADINQ ADKNI ADKPE ADMDM ADOAH ADOXG ADRFC ADTPH ADURQ ADYFF ADYOE ADYPR ADZKW AEAQA AEBTG AEEQQ AEFIE AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETLH AEVLU AEVTX AEXYK AFEXP AFGCZ AFKRA AFLOW AFNRJ AFQWF AFRAH AFWTZ AFYQB AFZKB AGAYW AGDGC AGGBP AGGDS AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AI. AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AJZVZ AKMHD AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMTXH AMXSW AMYLF AMYQR AOCGG AOSHJ ARMRJ ASPBG AVWKF AXYYD AZFZN AZQEC B-. BA0 BBNVY BBWZM BDATZ BENPR BGLVJ BGNMA BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CSCUP DDRTE DL5 DNIVK DPUIP DU5 DWQXO EBD EBLON EBS EIOEI EJD EMB EMOBN EN4 EPAXT ESBYG ESTFP F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNUQQ GNWQR GQ6 GQ7 GQ8 GXS HCIFZ HF~ HG5 HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IHE IJ- IKXTQ ITM IWAJR IXC IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KOW KPH L6V LAK LK8 LLZTM M0L M1P M2P M4Y M7P M7S MA- ML0 N2Q N9A NB0 NDZJH NEJ NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM OVD P0- P19 P2P PF0 PQQKQ PROAC PSQYO PT4 PT5 PTHSS Q2X QOK QOR QOS R4E R89 R9I RHV RNI RNS ROL RPX RRX RSV RZC RZE RZK S16 S1Z S26 S27 S28 S3A S3B SAP SBL SBY SCLPG SDH SDM SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZN T13 T16 TEORI TSG TSK TSV TUC U2A U9L UG4 UKHRP UNUBA UOJIU UTJUX UZXMN VC2 VFIZW VH1 W23 W48 W4F WJK WK6 WK8 XFK Y6R YLTOR Z45 Z5O Z7U Z7V Z7W Z7X Z7Y Z82 Z83 Z86 Z87 Z88 Z8O Z8P Z8Q Z8R Z8S Z8V Z8W Z91 Z92 ZMTXR ZOVNA ~02 ~EX ~KM AACDK AAEOY AAHBH AAJBT AAQLM AASML ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGQEE AIGIU ALIPV CGR CUY CVF ECM EIF NPM AAYXX AGRTI CITATION H13 7QL 7QR 7T7 7TB 7U5 7XB 8FD 8FK C1K FR3 K9. L7M M7N P64 PQEST PQUKI PRINS Q9U 7X8 |
ID | FETCH-LOGICAL-c375t-1382d68116eb9c5a4a61fd971774a141432c7b1f6a7c2942c6fba84d15c665f73 |
IEDL.DBID | 7X7 |
ISSN | 0141-5492 |
IngestDate | Fri Aug 16 05:11:29 EDT 2024 Fri Sep 13 06:43:26 EDT 2024 Thu Sep 12 16:25:47 EDT 2024 Sun Jun 23 00:31:09 EDT 2024 Sat Dec 16 12:09:19 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 8 |
Keywords | Whey permeate Ethanol genes Lactose fermentation LAC genes |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c375t-1382d68116eb9c5a4a61fd971774a141432c7b1f6a7c2942c6fba84d15c665f73 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0000-0003-2788-4187 |
PMID | 33937967 |
PQID | 2548029959 |
PQPubID | 54166 |
PageCount | 10 |
ParticipantIDs | proquest_miscellaneous_2521495388 proquest_journals_2548029959 crossref_primary_10_1007_s10529_021_03136_8 pubmed_primary_33937967 springer_journals_10_1007_s10529_021_03136_8 |
PublicationCentury | 2000 |
PublicationDate | 2021-08-01 |
PublicationDateYYYYMMDD | 2021-08-01 |
PublicationDate_xml | – month: 08 year: 2021 text: 2021-08-01 day: 01 |
PublicationDecade | 2020 |
PublicationPlace | Dordrecht |
PublicationPlace_xml | – name: Dordrecht – name: Netherlands |
PublicationTitle | Biotechnology letters |
PublicationTitleAbbrev | Biotechnol Lett |
PublicationTitleAlternate | Biotechnol Lett |
PublicationYear | 2021 |
Publisher | Springer Netherlands Springer Nature B.V |
Publisher_xml | – name: Springer Netherlands – name: Springer Nature B.V |
References | Sellick, Campbell, Reece (CR22) 2008; 269 Ryan, Walsh (CR20) 2016; 15 Escalante-Chong, Savir, Carroll, Ingraham, Wang, Marx, Springer (CR5) 2015; 112 Zou, Guo, Shen, Dong, Zhang, Xiao (CR27) 2013; 40 CR14 Lee, Hong, Jung, Ha, Yu, Koo, Park, Seo, Kweon, Park, Jin (CR12) 2011; 108 Jung, Park (CR10) 2005; 27 Soto, Fernandez, Vicente-Soler, Cansado, Gacto (CR24) 1999; 65 Akbas, Stark (CR1) 2016; 43 Crowe, Crowe, Chapman (CR3) 1984; 223 Ribeiro, Gombert, Teixeira, Domingues (CR17) 2007; 131 Rivas, Prazeres, Carvalho, Carvalho, Beltrán (CR18) 2010; 58 Gomes, Pataro, Guerra, Neves, Corra, Moreira, Rosa (CR6) 2002; 48 Ostergaard, Olsson, Johnston, Nielsen (CR15) 2000; 18 Quarterman, Skerker, Feng, Liu, Zhao, Arkin, Jin (CR16) 2016; 229 Saini, Beniwal, Kokkiligadda, Vij (CR21) 2017; 62 Rubio-Texeira, Castrillo, Adam, Ugalde, Polaina (CR19) 1998; 14 Zheng, Xu, Johnston (CR26) 1997; 272 Guimarães, François, Parrou, Teixeira, Domingues (CR8) 2008; 74 Guimarães, Teixeira, Domingues (CR9) 2010; 28 Nehlin, Carlberg, Ronne (CR13) 1991; 10 Domingues, Teixeira, Lima (CR4) 1999; 51 Yadav, Yan, Pilli, Kumar, Tyagi, Surampalli (CR25) 2015; 33 Bro, Knudsen, Regenberg, Olsson, Nielsen (CR2) 2005; 71 Kokkiligadda, Beniwal, Saini, Vij (CR11) 2016; 179 Ribeiro, Leão, Morais, Rosa, Panek (CR28) 1999; 75 Silva, Guimarães, Teixeira, Domingues (CR23) 2010; 37 Gueldener, Heinisch, Koehler, Voss, Hegemann (CR7) 2002; 30 MJS Ribeiro (3136_CR28) 1999; 75 PM Guimarães (3136_CR9) 2010; 28 3136_CR14 U Gueldener (3136_CR7) 2002; 30 CA Sellick (3136_CR22) 2008; 269 J Rivas (3136_CR18) 2010; 58 JH Crowe (3136_CR3) 1984; 223 J Zou (3136_CR27) 2013; 40 W Zheng (3136_CR26) 1997; 272 MP Ryan (3136_CR20) 2016; 15 S Ostergaard (3136_CR15) 2000; 18 J Quarterman (3136_CR16) 2016; 229 C Bro (3136_CR2) 2005; 71 L Domingues (3136_CR4) 1999; 51 YJ Jung (3136_CR10) 2005; 27 T Soto (3136_CR24) 1999; 65 PM Guimarães (3136_CR8) 2008; 74 O Ribeiro (3136_CR17) 2007; 131 FC Gomes (3136_CR6) 2002; 48 JS Yadav (3136_CR25) 2015; 33 MY Akbas (3136_CR1) 2016; 43 A Kokkiligadda (3136_CR11) 2016; 179 M Rubio-Texeira (3136_CR19) 1998; 14 R Escalante-Chong (3136_CR5) 2015; 112 KS Lee (3136_CR12) 2011; 108 JO Nehlin (3136_CR13) 1991; 10 AC Silva (3136_CR23) 2010; 37 P Saini (3136_CR21) 2017; 62 |
References_xml | – volume: 40 start-page: 353 year: 2013 end-page: 363 ident: CR27 article-title: Construction of lactose-consuming Saccharomyces cerevisiae for lactose fermentation into ethanol fuel publication-title: J Ind Microbiol Biotechnol doi: 10.1007/s10295-012-1227-5 contributor: fullname: Xiao – volume: 14 start-page: 827 issue: 9 year: 1998 end-page: 837 ident: CR19 article-title: Highly efficient assimilation of lactose by a metabolically engineered strain of Saccharomyces cerevisiae publication-title: Yeast doi: 10.1002/(SICI)1097-0061(19980630)14:9<827::AID-YEA281>3.0.CO;2-N contributor: fullname: Polaina – volume: 27 start-page: 1855 issue: 23–24 year: 2005 end-page: 1859 ident: CR10 article-title: Antisense-mediated inhibition of acid trehalase ( ) gene expression promotes ethanol fermentation and tolerance in Saccharomyces cerevisiae publication-title: Biotechnol Lett doi: 10.1007/s10529-005-3910-3 contributor: fullname: Park – volume: 131 start-page: 20 year: 2007 end-page: 26 ident: CR17 article-title: Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus publication-title: J Biotechnol doi: 10.1016/j.jbiotec.2007.05.027 contributor: fullname: Domingues – volume: 65 start-page: 2020 issue: 5 year: 1999 end-page: 2024 ident: CR24 article-title: Accumulation of trehalose by overexpression of tps1, coding for trehalose-6-phosphate synthase, causes increased resistance to multiple stresses in the fission yeast Schizosaccharomyces pombe publication-title: Appl Environ Microb doi: 10.1128/AEM.65.5.2020-2024.1999 contributor: fullname: Gacto – volume: 15 start-page: 479 year: 2016 end-page: 498 ident: CR20 article-title: The biotechnological potential of whey publication-title: Rev Environ Sci Biol doi: 10.1007/s11157-016-9402-1 contributor: fullname: Walsh – volume: 28 start-page: 375 year: 2010 end-page: 384 ident: CR9 article-title: Fermentation of lactose to bio-ethanol by yeasts as part of integrated solutions for the valorisation of cheese whey publication-title: Biotechnol Adv doi: 10.1016/j.biotechadv.2010.02.002 contributor: fullname: Domingues – ident: CR14 – volume: 75 start-page: 245 issue: 3 year: 1999 end-page: 251 ident: CR28 article-title: Trehalose accumulation by tropical yeast strains submitted to stress conditions publication-title: Antio Leeuw Int J G doi: 10.1023/A:1001806012566 contributor: fullname: Panek – volume: 30 start-page: e23 year: 2002 ident: CR7 article-title: A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast publication-title: Nucleic Acids Res doi: 10.1093/nar/30.6.e23 contributor: fullname: Hegemann – volume: 229 start-page: 13 year: 2016 end-page: 21 ident: CR16 article-title: Rapid and efficient galactose fermentation by engineered Saccharomyces cerevisiae publication-title: J Biotechnol doi: 10.1016/j.jbiotec.2016.04.041 contributor: fullname: Jin – volume: 58 start-page: 7871 year: 2010 end-page: 7877 ident: CR18 article-title: Treatment of cheese whey wastewater: combined coagulation-flocculation and aerobic biodegradation publication-title: J Agric Food Chem doi: 10.1021/jf100602j contributor: fullname: Beltrán – volume: 62 start-page: 69 year: 2017 end-page: 79 ident: CR21 article-title: Evolutionary adaptation of Kluyveromyces marxianus strain for efficient conversion of whey lactose to bioethanol publication-title: Process Biochem doi: 10.1016/j.procbio.2017.07.013 contributor: fullname: Vij – volume: 108 start-page: 621 issue: 3 year: 2011 end-page: 631 ident: CR12 article-title: Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering publication-title: Biotechnol Bioeng doi: 10.1002/bit.22988 contributor: fullname: Jin – volume: 43 start-page: 1593 issue: 11 year: 2016 end-page: 1609 ident: CR1 article-title: Recent trends in bioethanol production from food processing byproducts publication-title: J Ind Microbiol Biot doi: 10.1007/s10295-016-1821-z contributor: fullname: Stark – volume: 51 start-page: 621 year: 1999 end-page: 626 ident: CR4 article-title: Construction of a flocculent Saccharomyces cerevisiae fermenting lactose publication-title: Appl Microbiol Biotechnol doi: 10.1007/s002530051441 contributor: fullname: Lima – volume: 48 start-page: 399 issue: 5 year: 2002 end-page: 406 ident: CR6 article-title: Physiological diversity and trehalose accumulation in Schizosaccharomyces pombe strains isolated from spontaneous fermentations during the production of the artisanal Brazilian cachaca publication-title: Can J Microbiol doi: 10.1139/w02-032 contributor: fullname: Rosa – volume: 18 start-page: 1283 year: 2000 end-page: 1286 ident: CR15 article-title: Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network publication-title: Nat Biotechnol doi: 10.1038/82400 contributor: fullname: Nielsen – volume: 37 start-page: 973 year: 2010 end-page: 982 ident: CR23 article-title: Fermentation of deproteinized cheese whey powder solutions to ethanol by engineered Saccharomyces cerevisiae: effect of supplementation with corn steep liquor and repeated-batch operation with biomass recycling by flocculation publication-title: J Ind Microbiol Biotechnol doi: 10.1007/s10295-010-0748-z contributor: fullname: Domingues – volume: 272 start-page: 30350 year: 1997 end-page: 30355 ident: CR26 article-title: The cysteine–peptidase bleomycin hydrolase is a member of the galactose regulon in yeast publication-title: J Biol Chem doi: 10.1074/jbc.272.48.30350 contributor: fullname: Johnston – volume: 74 start-page: 1748 issue: 6 year: 2008 end-page: 1756 ident: CR8 article-title: Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant publication-title: Appl Environ Microbiol doi: 10.1128/AEM.00186-08 contributor: fullname: Domingues – volume: 33 start-page: 756 year: 2015 end-page: 774 ident: CR25 article-title: Cheese whey: a potential resource to transform into bioprotein, functional/nutritional proteins and bioactive peptides publication-title: Biotechnol Adv doi: 10.1016/j.biotechadv.2015.07.002 contributor: fullname: Surampalli – volume: 10 start-page: 3373 year: 1991 end-page: 3377 ident: CR13 article-title: Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response publication-title: EMBO J doi: 10.1002/j.1460-2075.1991.tb04901.x contributor: fullname: Ronne – volume: 112 start-page: 1636 issue: 5 year: 2015 end-page: 1641 ident: CR5 article-title: Galactose metabolic genes in yeast respond to a ratio of galactose and glucose publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1418058112 contributor: fullname: Springer – volume: 71 start-page: 6465 issue: 11 year: 2005 end-page: 6472 ident: CR2 article-title: Improvement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: example of transcript analysis as a tool in inverse metabolic engineering publication-title: Appl Environ Microbiol doi: 10.1128/AEM.71.11.6465-6472.2005 contributor: fullname: Nielsen – volume: 269 start-page: 111 year: 2008 end-page: 150 ident: CR22 article-title: Galactose metabolism in yeast-structure and regulation of the leloir pathway enzymes and the genes encoding them publication-title: Int Rev Cell Mol Biol doi: 10.1016/S1937-6448(08)01003-4 contributor: fullname: Reece – volume: 223 start-page: 701 issue: 4637 year: 1984 end-page: 703 ident: CR3 article-title: Preservation of membranes in anhydrobiotic organisms: the role of trehalose publication-title: Science doi: 10.1126/science.223.4637.701 contributor: fullname: Chapman – volume: 179 start-page: 1469 year: 2016 end-page: 1484 ident: CR11 article-title: Utilization of cheese whey using synergistic immobilization of beta-galactosidase and Saccharomyces cerevisiae cells in dual matrices publication-title: Appl Biochem Biotechnol doi: 10.1007/s12010-016-2078-8 contributor: fullname: Vij – volume: 229 start-page: 13 year: 2016 ident: 3136_CR16 publication-title: J Biotechnol doi: 10.1016/j.jbiotec.2016.04.041 contributor: fullname: J Quarterman – volume: 51 start-page: 621 year: 1999 ident: 3136_CR4 publication-title: Appl Microbiol Biotechnol doi: 10.1007/s002530051441 contributor: fullname: L Domingues – volume: 223 start-page: 701 issue: 4637 year: 1984 ident: 3136_CR3 publication-title: Science doi: 10.1126/science.223.4637.701 contributor: fullname: JH Crowe – volume: 10 start-page: 3373 year: 1991 ident: 3136_CR13 publication-title: EMBO J doi: 10.1002/j.1460-2075.1991.tb04901.x contributor: fullname: JO Nehlin – volume: 18 start-page: 1283 year: 2000 ident: 3136_CR15 publication-title: Nat Biotechnol doi: 10.1038/82400 contributor: fullname: S Ostergaard – volume: 40 start-page: 353 year: 2013 ident: 3136_CR27 publication-title: J Ind Microbiol Biotechnol doi: 10.1007/s10295-012-1227-5 contributor: fullname: J Zou – volume: 27 start-page: 1855 issue: 23–24 year: 2005 ident: 3136_CR10 publication-title: Biotechnol Lett doi: 10.1007/s10529-005-3910-3 contributor: fullname: YJ Jung – volume: 14 start-page: 827 issue: 9 year: 1998 ident: 3136_CR19 publication-title: Yeast doi: 10.1002/(SICI)1097-0061(19980630)14:9<827::AID-YEA281>3.0.CO;2-N contributor: fullname: M Rubio-Texeira – volume: 112 start-page: 1636 issue: 5 year: 2015 ident: 3136_CR5 publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1418058112 contributor: fullname: R Escalante-Chong – volume: 75 start-page: 245 issue: 3 year: 1999 ident: 3136_CR28 publication-title: Antio Leeuw Int J G doi: 10.1023/A:1001806012566 contributor: fullname: MJS Ribeiro – volume: 71 start-page: 6465 issue: 11 year: 2005 ident: 3136_CR2 publication-title: Appl Environ Microbiol doi: 10.1128/AEM.71.11.6465-6472.2005 contributor: fullname: C Bro – volume: 43 start-page: 1593 issue: 11 year: 2016 ident: 3136_CR1 publication-title: J Ind Microbiol Biot doi: 10.1007/s10295-016-1821-z contributor: fullname: MY Akbas – volume: 269 start-page: 111 year: 2008 ident: 3136_CR22 publication-title: Int Rev Cell Mol Biol doi: 10.1016/S1937-6448(08)01003-4 contributor: fullname: CA Sellick – volume: 74 start-page: 1748 issue: 6 year: 2008 ident: 3136_CR8 publication-title: Appl Environ Microbiol doi: 10.1128/AEM.00186-08 contributor: fullname: PM Guimarães – volume: 179 start-page: 1469 year: 2016 ident: 3136_CR11 publication-title: Appl Biochem Biotechnol doi: 10.1007/s12010-016-2078-8 contributor: fullname: A Kokkiligadda – volume: 131 start-page: 20 year: 2007 ident: 3136_CR17 publication-title: J Biotechnol doi: 10.1016/j.jbiotec.2007.05.027 contributor: fullname: O Ribeiro – volume: 58 start-page: 7871 year: 2010 ident: 3136_CR18 publication-title: J Agric Food Chem doi: 10.1021/jf100602j contributor: fullname: J Rivas – ident: 3136_CR14 – volume: 30 start-page: e23 year: 2002 ident: 3136_CR7 publication-title: Nucleic Acids Res doi: 10.1093/nar/30.6.e23 contributor: fullname: U Gueldener – volume: 28 start-page: 375 year: 2010 ident: 3136_CR9 publication-title: Biotechnol Adv doi: 10.1016/j.biotechadv.2010.02.002 contributor: fullname: PM Guimarães – volume: 272 start-page: 30350 year: 1997 ident: 3136_CR26 publication-title: J Biol Chem doi: 10.1074/jbc.272.48.30350 contributor: fullname: W Zheng – volume: 62 start-page: 69 year: 2017 ident: 3136_CR21 publication-title: Process Biochem doi: 10.1016/j.procbio.2017.07.013 contributor: fullname: P Saini – volume: 37 start-page: 973 year: 2010 ident: 3136_CR23 publication-title: J Ind Microbiol Biotechnol doi: 10.1007/s10295-010-0748-z contributor: fullname: AC Silva – volume: 48 start-page: 399 issue: 5 year: 2002 ident: 3136_CR6 publication-title: Can J Microbiol doi: 10.1139/w02-032 contributor: fullname: FC Gomes – volume: 108 start-page: 621 issue: 3 year: 2011 ident: 3136_CR12 publication-title: Biotechnol Bioeng doi: 10.1002/bit.22988 contributor: fullname: KS Lee – volume: 15 start-page: 479 year: 2016 ident: 3136_CR20 publication-title: Rev Environ Sci Biol doi: 10.1007/s11157-016-9402-1 contributor: fullname: MP Ryan – volume: 65 start-page: 2020 issue: 5 year: 1999 ident: 3136_CR24 publication-title: Appl Environ Microb doi: 10.1128/AEM.65.5.2020-2024.1999 contributor: fullname: T Soto – volume: 33 start-page: 756 year: 2015 ident: 3136_CR25 publication-title: Biotechnol Adv doi: 10.1016/j.biotechadv.2015.07.002 contributor: fullname: JS Yadav |
SSID | ssj0009707 |
Score | 2.3906953 |
Snippet | Objectives
Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by... Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy... ObjectivesDevelopment of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by... OBJECTIVESDevelopment of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by... |
SourceID | proquest crossref pubmed springer |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 1607 |
SubjectTerms | Applied Microbiology Batch processes Batch processing Biochemistry Biomedical and Life Sciences Bioreactors Bioreactors - microbiology Biotechnology Catabolite repression Chemoreception Conversion Dairy industry Diploids Ethanol Ethanol - metabolism Fermentation Fermentation - genetics Fungal Proteins - genetics Galactose Gene silencing Genes Glucose Glucose - metabolism Hydrolase Kluyveromyces - genetics Lactose Lactose - genetics Lactose - metabolism Life Sciences Metabolic Engineering Metabolism Microbiology NTH1 protein Original Research Paper Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Whey Whey - metabolism Yeast Yeasts |
SummonAdditionalLinks | – databaseName: SpringerLink Journals (ICM) dbid: U2A link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1NT-MwEB2xICQ4rPhcunzISNwgEnb8lWO1AioEXKASt8hxYqhEU7RpD_z7nXHTFgQc9hglnkTzHHssv_cMcMJJmOeCTazSIZE2FEmWKolrHuMQfyy4o77i9k73-vL6UT0udNyR7D7bkYwD9TutmxJZQowCshvUif0BK1Q8UFfui-7Cade0GmnJE7Ifa5UyX8f4OBt9KjE_bY_GWedyA3625SLrTvHdhKWq3oLV6QGSb1uw_s5OcBte-7UfTUhi-8RaKjpriKCO1yQjYVfdGzasxoj7y6AZMqxX2TPRYSjaaNKwFzp8BxsFHK5bTVLNBjW7d57UWaPhG44qzEdqcDNw1Q70Ly8e_vSS9kSFxKdGjaPhYKkt57oqMq-cdJqHMsMlnZEOEyZT4U3Bg3bGi0wKr0PhrCy58lqrYNJdWK5HdbUHDKf18zJwgcsVL12pCovtjax45rgueejA6Syz-evUOCNfWCQTDjnikEccctuBg1ny8_YnanJBXnSCDNE6cDy_jd2f9jRcXWFi8BkRKbIWQ_yagjZ_XUpmf5k2HTibobgI_v23_P6_x_dhTcQeRaTAA1ge_51Uh1iojIuj2DH_AXva3Rs priority: 102 providerName: Springer Nature |
Title | Uncoupling glucose sensing from GAL metabolism for heterologous lactose fermentation in Saccharomyces cerevisiae |
URI | https://link.springer.com/article/10.1007/s10529-021-03136-8 https://www.ncbi.nlm.nih.gov/pubmed/33937967 https://www.proquest.com/docview/2548029959/abstract/ https://search.proquest.com/docview/2521495388 |
Volume | 43 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lj9MwEB4tu0KCA4LlVVhWRuIGUbHjV06oRX2IR4WASuUUOU4MlbZJIelh_z0zabIFreASK3LsWJ6xPbbn-wbgBSdgngs2skqHSNqQRUmsJO55jEP5o8Hd4is-LvR8Kd-t1OoI5j0Whtwq-zmxnajzytMZ-VAQMZkgdqyhy-gUwDfDN9ufEcWPonvWLpjGDTjhAs0K1GyzMgf6XdMBpyWPiJOsg890IDolkohcFYjHUEf27yXqmt157c60XYqmd-FOZ0Oy0V7o9-CoKE_h5j6q5OUp3P6DY_A-bJelr3aEu_3OOv90VpPXOr4TtoTNRh_YpmhQGS7W9YahEct-kI8M1VbtanZBEXmwUMA5vAMqlWxdsi_OE2Sr2lziVMN86y9cr13xAJbTyde386gLsxD52KimZSHMteVcF1nilZNO85AnuM8z0mGHyVh4k_GgnfEikcLrkDkrc6681iqY-CEcl1VZPAaGa_3rPHCBexgvXa4yi-WNLHjiuM55GMDLvmfT7Z5NIz3wJpMcUpRD2sohtQM46zs_7UZWnR70YADPr7JxTNBFhysL7Bj8RrR-sxareLQX2tXvYmIATLQZwKteiofK_92WJ_9vy1O4JVoNIs_AMzhufu2KZ2itNNl5q4j4tNPZOZyMpuPxgtLZt_cTTMeTxafPmLsUo9_lOerp |
link.rule.ids | 315,786,790,12083,12792,21416,27957,27958,31754,31755,33408,33409,33779,33780,41116,41558,42185,42627,43345,43635,43840,52146,52269,74102,74392,74659 |
linkProvider | ProQuest |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lj9MwEB7BIgQcECyvwgJG4gbWYsevnNAKsRTo7oWt1FvkODFU2iaFtIf998y47ha0gmOU2LFm_Bjb3_cNwGtBxDwfHXfaRK5crHlZaIV7HuvR_xhwJ37FyakZT9WXmZ7lA7chwyq3c2KaqJs-0Bn5oSRhMknqWO-XPzlljaLb1ZxC4zrcUEWhCNJnZ3YnumszXVoJTkpkmTSTqXNalpwACqReaLj7e2G6Em1euSlNC9DxPbibI0d2tHH1fbjWdvtwc5NL8mIf7vyhLPgAltMu9Gti235nGZXOBsKq4zMxStinowlbtCvsAufzYcEwdGU_CBlDtfXrgZ1THh4sFHHmzvSkjs079s0HImr1iwucYFhIKOFh7tuHMD3-ePZhzHNyBR4Kq1dJe7AxTgjT1mXQXnkjYlPi7s4qjwZThQy2FtF4G2SpZDCx9k41QgdjdLTFI9jr-q59AgxX-HdNFBJ3LkH5RtcOy1vVitIL04g4gjdby1bLjYZGtVNLJj9U6Icq-aFyIzjYGr_K42modt4fwavL1zgS6HrDdy0aBr-RCS3rsIrHG6dd_q4g3b_S2BG83XpxV_m_2_L0_215CbfGZyeTavL59OszuC1TbyJs4AHsrX6t2-cYr6zqF6lT_gb7CuOj |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwEB1BKxAcKihfSwsYiRtYrR1_5YTa0qVAWVXASr1FjhPDSt1kIbuH_ntmst4uqIJjlNiJPOPxOH7vDcArQcQ8Hx132kSuXCx5nmmFex7r0f6YcPf8is8jczJWH8_1ecI_dQlWuYqJfaCu2kD_yPckCZNJUsfaiwkWcfZu-Hb2k1MFKTppTeU0bsKmVUajh28eHo_OvqwleG0iTyvBSZcsUWgSkU7LnBNcgbQMDXd_L1PXcs9r56b9cjS8B1spj2QHS8Pfhxt1sw23lpUlL7fh7h86gw9gNm5CuyDu7XeWMOqsI-Q6XhO_hL0_OGXTeo4OcTHppgwTWfaDcDLUW7vo2AVV5cFGEeN4Iis1bNKwrz4QbaudXmK4YaHHDHcTXz-E8fD429EJT6UWeMisnvdKhJVxQpi6zIP2yhsRqxz3elZ5HDCVyWBLEY23QeZKBhNL71QldDBGR5s9go2mbeonwHC936-ikLiPCcpXunTY3qpa5F6YSsQBvF6NbDFbKmoUa-1kskOBdih6OxRuALurwS_S7OqKtS8M4OXVbZwXdNjhmxoHBp-RPXbWYRePl0a7el1GKoC5sQN4s7LiuvN_f8vT_3_LC7iNHlmcfhh92oE7sncmAgruwsb816J-hsnLvHyevPI30ebpRg |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Uncoupling+glucose+sensing+from+GAL+metabolism+for+heterologous+lactose+fermentation+in+Saccharomyces+cerevisiae&rft.jtitle=Biotechnology+letters&rft.au=Zou+Jing&rft.au=Chen%2C+Xiaohui&rft.au=Hu+Yinghong&rft.au=Xiao+Dongguang&rft.date=2021-08-01&rft.pub=Springer+Nature+B.V&rft.issn=0141-5492&rft.eissn=1573-6776&rft.volume=43&rft.issue=8&rft.spage=1607&rft.epage=1616&rft_id=info:doi/10.1007%2Fs10529-021-03136-8&rft.externalDBID=HAS_PDF_LINK |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0141-5492&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0141-5492&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0141-5492&client=summon |